Ultrasensitive sensing of Androctonus australis hector scorpion venom toxins in biological fluids using an electrochemical graphene quantum dots/nanobody-based platform

Talanta
Abdelmoneim MarsNoureddine Raouafi

Abstract

Rapid and sensitive detection of low levels of scorpion venom toxins in biological fluids is of tremendous importance for decision-taking in cases of envenomation by scorpions stings. In Tunisia, at least 1200 severe envenomation cases by Androctonus australis hector (Aah) scorpion stings were reported annually. In this work, we report on a novel electrochemical immuno-sandwich to detect the Aah50 toxic fraction within the Aah scorpion venom using the bispecific nanobody format specially designed to highly recognize and neutralize the two most toxic molecules in the AahG50 venom fraction (i.e. AahI and AahII toxins), graphene quantum dots (GQDs) constructed on the surface carbon screen-printed electrodes. Hydroquinone/H2O2/peroxidase system was used to amplify the current in order to achieve the detection of low levels of AahG50. Electrochemical studies revealed a high sensitivity toward the AahG50 with a sensitivity of 18.2 nA mL pg-1 and a picomolar limit of detection as low as 0.55 pg mL-1. The platform exhibits very good metrological performances such as repeatability, reproducibility, selectivity and long storage stability. Matrix effect was found to be insignificant as demonstrated by assays performed in human blood serum...Continue Reading

References

Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·S PukrittayakameeD Bunnag
Jan 1, 1982·Toxicon : Official Journal of the International Society on Toxinology·M GoyffonN Broglio
Dec 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·C Chávez-OlórteguiC R Diniz
Sep 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·M Dehesa-Dávila, L D Possani
Jul 14, 1999·Biologicals : Journal of the International Association of Biological Standardization·M N KrifiK Dellagi
Aug 23, 2002·Biosensors & Bioelectronics·Z Emmanuel SelvanayagamL C Ho
Jun 27, 2008·Acta Tropica·J-P Chippaux, M Goyffon
Oct 22, 2008·Toxicon : Official Journal of the International Society on Toxinology·Mounir BouazizNoureddine Rekik
Jan 24, 2009·Biosensors & Bioelectronics·Soundappan ThiagarajanShen-Ming Chen
Apr 23, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Issam HmilaSerge Muyldermans
Jun 10, 2011·Journal of the American Chemical Society·Vinay GuptaSuresh Chand
Jan 28, 2015·ACS Nano·Qing ZhangShuit-Tong Lee

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Citations

Jun 20, 2019·Chemical Society Reviews·Khadijeh NekoueianSabine Szunerits
Dec 9, 2020·Chemistry, an Asian Journal·Jiameng WangHongyuan Yan
Oct 16, 2020·International Journal of Biological Macromolecules·Erika A Bastos-SoaresCarla Freire C Fernandes
Jan 5, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Poorvisha Ravi, Muthupandian Ganesan

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