Ultrasensitive Virion Immunoassay Platform with Dual-Modality Based on a Multifunctional Aggregation-Induced Emission Luminogen

ACS Nano
Ling-Hong XiongBen Zhong Tang

Abstract

Sensitive and accurate detection of highly contagious virus is urgently demanded for disease diagnosis and treatment. Herein, based on a multifunctional aggregation-induced emission luminogen (AIEgen), a dual-modality readout immunoassay platform for ultrasensitive detection of viruses has been successfully demonstrated. The platform is relied on virions immuno-bridged enzymatic hydrolysis of AIEgen, accompanying with the in situ formation of highly emissive AIE aggregates and shelling of silver on gold nanoparticles. As a result, robust turn-on fluorescence and naked-eye discernible plasmonic colorimetry composed dual-signal is achieved. By further taking advantage of effective immunomagnetic enrichment, EV71 virions, as an example, can be specifically detected with a limit of detection down to 1.4 copies/μL under fluorescence modality. Additionally, semiquantitative discerning of EV71 virions is realized in a broad range from 1.3 × 103 to 2.5 × 106 copies/μL with the naked eye. Most importantly, EV71 virions in 24 real clinical samples are successfully diagnosed with 100% accuracy. Comparing to the gold standard polymerase chain reaction (PCR) assay, our immunoassay platform do not need complicated sample pretreatment and exp...Continue Reading

References

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May 2, 2014·ACS Nano·Ling-Hong XiongDai-Wen Pang
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May 10, 2018·Journal of the American Chemical Society·Xuewen HeBen Zhong Tang

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Citations

Jan 3, 2019·Chemistry : a European Journal·Sheng XieBen Zhong Tang
Oct 9, 2020·Biosensors & Bioelectronics·Akhilesh Babu GanganboinaEnoch Y Park
Feb 10, 2021·Medicinal Research Reviews·Xianlong ZhangTony D James
Oct 31, 2020·Frontiers in Immunology·Haibo WuYuanzeng Min
Feb 16, 2021·Advanced Materials·Eunjung KimSeungjoo Haam
Mar 9, 2021·Frontiers in Immunology·Zongwei LiuWeijiang Guan
Apr 10, 2021·Topics in Current Chemistry·Harini A Perera, Mingdi Yan
Jan 31, 2020·Journal of the American Chemical Society·Xuewen HeBen Zhong Tang
Jun 18, 2021·Advanced Materials·Jing ZhangBen Zhong Tang
Jun 14, 2021·Biosensors & Bioelectronics·Yaofeng ZhouYonghua Xiong
Nov 9, 2019·Analytical Chemistry·Mingyuan DuZhike He
Aug 23, 2019·Accounts of Chemical Research·Dong Wang, Ben Zhong Tang
May 31, 2019·Analytical Chemistry·Lei JiaoChengzhou Zhu
Oct 16, 2019·ACS Applied Materials & Interfaces·Xiaolun PengShengfu Wang
Sep 19, 2019·ACS Applied Materials & Interfaces·Piaopiao ChenJia Geng
Sep 10, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Jianxin WangSeungpyo Hong
Oct 9, 2021·Advanced Materials·Zhaowei ZhangUtkan Demirci
Jan 7, 2022·Journal of Agricultural and Food Chemistry·Leina DouZhanhui Wang

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