Ultrasmall Superparamagnetic Iron Oxide Labeled Silk Fibroin/Hydroxyapatite Multifunctional Scaffold Loaded With Bone Marrow-Derived Mesenchymal Stem Cells for Bone Regeneration

Frontiers in Bioengineering and Biotechnology
Qin LiuYikai Xu

Abstract

Numerous tissue-engineered constructs have been investigated as bone scaffolds in regenerative medicine. However, it remains challenging to non-invasively monitor the biodegradation and remodeling of bone grafts after implantation. Herein, silk fibroin/hydroxyapatite scaffolds incorporated with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles were successfully synthesized, characterized, and implanted subcutaneously into the back of nude mice. The USPIO labeled scaffolds showed good three-dimensional porous structures and mechanical property, thermal stability for bone repair. After loaded with bone marrow-derived mesenchymal stem cells (BMSCs), the multifunctional scaffolds promoted cell adhesion and growth, and facilitated osteogenesis by showing increased levels of alkaline phosphatase activity and up-regulation of osteoblastic genes. Furthermore, in vivo quantitative magnetic resonance imaging (MRI) results provided valuable information on scaffolds degradation and bone formation simultaneously, which was further confirmed by computed tomography and histological examination. These findings demonstrated that the incorporation of USPIO into BMSCs-loaded multifunctional scaffold system could be feasible to noninva...Continue Reading

References

Jan 25, 2003·Tissue Engineering·Ed H M HartmanPaul H M Spauwen
Jul 29, 2010·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Hai-Ling Margaret ChengWalid A Farhat
Mar 29, 2011·Acta Biomaterialia·Hongjian Zhou, Jaebeom Lee
May 23, 2012·Methods in Molecular Biology·Susanne GrässelZsuzsa Jenei-Lanzl
Sep 4, 2012·Trends in Biotechnology·Susmita BoseAmit Bandyopadhyay
Nov 28, 2012·Advanced Healthcare Materials·Naresh Kasoju, Utpal Bora
Jun 19, 2013·Biomaterials·Alyssa A AppelEric M Brey
Feb 4, 2014·The Journal of Clinical Investigation·Janet L Crane, Xu Cao
Feb 27, 2014·Advanced Functional Materials·Marianne E MertensTwan Lammers
Jul 12, 2014·Tissue Engineering. Part B, Reviews·Seung Yun NamStanislav Y Emelianov
Mar 21, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Graham G WalmsleyDerrick C Wan
Sep 12, 2015·Acta Biomaterialia·Johanna MelkeSandra Hofmann
Jan 21, 2016·Advanced Healthcare Materials·Neil M EisensteinLiam M Grover
May 21, 2016·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Deana HaralampievaSimon M Ametamey
Sep 1, 2016·ACS Applied Materials & Interfaces·Zhaozhao DingDavid L Kaplan
Oct 23, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Hyung-Mun YunEun-Cheol Kim
Mar 13, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Sibaram BeheraSubhas C Kundu
Jun 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Livia RosetiBrunella Grigolo
Jun 26, 2017·Cells, Tissues, Organs·Ahmad OryanMohamadreza Baghaban Eslaminejad
Jul 15, 2017·Journal of Biomaterials Science. Polymer Edition·Vahideh Raeisdasteh HokmabadRoya Salehi
Oct 11, 2017·Biotechnology Advances·Mehdi FarokhiDavid L Kaplan
Jan 13, 2018·Journal of Tissue Engineering and Regenerative Medicine·Shuying HuHockin H K Xu
May 13, 2018·Materials Science & Engineering. C, Materials for Biological Applications·Dakun MaWenjie Dai
Mar 13, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Koythatta Meethaleveetil SajeshManzoor Koyakutty
Nov 26, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Xiangsheng WangXiansong Wang

❮ Previous
Next ❯

Citations

Nov 17, 2020·Current Opinion in Psychiatry·Nady BraidyPerminder S Sachdev
Jun 23, 2021·Chemical Society Reviews·Xianwen WangLiang Cheng
Sep 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuying HuNing Gu

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
PCR

Software Mentioned

Image J
SPSS
Imalytics Preclinical Software

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.