Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes

International Journal of Biological Macromolecules
Jixian ZhangYuqing Duan

Abstract

Some studies have demonstrated that polysaccharides are the chief ingredient of Lentinus edodes. To extract the useful polysaccharides from Lentinus edodes, we used a novel extraction method, which was named ultrasonic-enhanced subcritical water extraction by two steps (USWE). Four independent factors (extraction temperature, extraction time, liquid-to-solid ratio, and ultrasonic power) were studied. A Box-Behnken design (BBD) was applied to optimize the USWE condition based on the results of single-factor tests. The maximum extraction rate of polysaccharides was 17.34%. In addition, extracted polysaccharides at optimal condition were compared with subcritical water extraction (SWE), ultrasonic extraction (UE), and hot water extraction (HWE) samples. The antioxidant against 2,2‑diphenyl‑1‑picryl‑hydrazyl (DPPH) and reducing power were also evaluated. The IC50 value for DPPH scavenging rate of HWE, SWE, UE, and USWE sample was 0.341, 0.237, 0.280, and 0.232 mg/mL, respectively. The USWE sample had the highest reducing power activity. The mole ratio of the monosaccharide composition of four samples was different. The results of AFM and SEM showed that the morphology of the four polysaccharides showed significant differences. Thes...Continue Reading

Citations

Dec 4, 2020·Journal of the Science of Food and Agriculture·Yang ZhangZhaowei Yan
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan ChengYu Yang
Jul 15, 2021·Critical Reviews in Food Science and Nutrition·He LiuDanshi Zhu
Jul 20, 2021·International Journal of Biological Macromolecules·Xiomara López-LegardaFreimar Segura-Sánchez

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