PMID: 3768916Sep 1, 1986Paper

Ultrasonic irradiation of bacterial polysaccharides. Characterization of the depolymerized products and some applications of the process

Carbohydrate Research
S C SzuJ B Robbins

Abstract

Ultrasonic irradiation (u.i.) has been used to depolymerize biopolymers including DNA, dextran, and the Vi capsular polysaccharide from Citrobacter freundii. Representative bacterial polysaccharides were subjected to u.i. and the effect of this energy upon their molecular weight and chemical structure was characterized. U.i. depolymerized a neutral polysaccharide (dextran) and acidic polysaccharides containing either a phosphoric diester linkage [Haemophilus influenzae type b (Hib) and pneumococcus types 6A and 6B] or a uronic acid moiety (pneumococcus type 9N). Prolonged u.i. depolymerized all the polysaccharides to a finite and similar molecular mass (approximately 50 000 daltons). The rate of depolymerization induced by u.i. depended on the viscosity of the solvent and the concentration of the polysaccharide. 13C-N.m.r. data of the native Hib polysaccharide and its depolymerized products indicated that u.i. did not alter the chemical structure of the repeating units. Determination of the monophosphate terminal residues by 31P-n.m.r. spectroscopy and of the reducing end groups by the Park-Johnson reaction indicated that both the phosphoric diester and the glycosidic linkages were cleaved. The Vi polysaccharide, prepared as an...Continue Reading

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Citations

Jun 7, 2005·Ultrasonics Sonochemistry·M VodenicarováA Ebringerová
Apr 19, 2000·Ultrasonics Sonochemistry·D MislovicováE Machová
Sep 10, 1999·Microbiology and Immunology·M SinghH Vohra
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Somporn MoonmangmeeKazunobu Matsushit
Feb 6, 2017·International Journal of Biological Macromolecules·Qilin TangHui Li
Apr 27, 2016·Infection and Immunity·Guillaume Goyette-DesjardinsMariela Segura
Apr 20, 2005·Annals of the New York Academy of Sciences·John B Robbins, Rachel Schneerson
Jan 27, 2021·Ultrasonics Sonochemistry·Lena Rebecca LarsenFabian Weber
Oct 1, 1989·Infection and Immunity·E M DanielsJ B Robbins

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