Ultrasound and microbubble induced release from intracellular compartments

BMC Biotechnology
Farah HusseinRaffi Karshafian

Abstract

Ultrasound and microbubbles (USMB) have been shown to enhance the intracellular uptake of molecules, generally thought to occur as a result of sonoporation. The underlying mechanism associated with USMB-enhanced intracellular uptake such as membrane disruption and endocytosis may also be associated with USMB-induced release of cellular materials to the extracellular milieu. This study investigates USMB effects on the molecular release from cells through membrane-disruption and exocytosis. USMB induced the release of 19% and 67% of GFP from the cytoplasm in viable and non-viable cells, respectively. Tfn release from early/recycling endosomes increased by 23% in viable cells upon USMB treatment. In addition, the MFI of LAMP-1 antibody increased by 50% in viable cells, suggesting USMB-stimulated lysosome exocytosis. In non-viable cells, labeling of LAMP-1 intracellular structures in the absence of cell permeabilization by detergents suggests that USMB-induced cell death correlates with lysosomal permeabilization. In conclusion, USMB enhanced the molecular release from the cytoplasm, lysosomes, and early/recycling endosomes.

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Citations

Nov 29, 2017·Bioscience Reports·Anthony DelalandeChantal Pichon
May 15, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Valerio PerenoEleanor Stride
Dec 12, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Jean-Michel Escoffre, Ayache Bouakaz

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy

Software Mentioned

ImageJ

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