Ultrasound and microbubble-targeted delivery of small interfering RNA into primary endothelial cells is more effective than delivery of plasmid DNA

Ultrasound in Medicine & Biology
Lynda J M JuffermansLeo E Deelman

Abstract

Ultrasound and microbubble-targeted delivery (UMTD) is a promising non-viral technique for genetic-based therapy. We found that UMTD of small interfering RNA (siRNA) is more effective than delivery of plasmid DNA (pDNA). UMTD (1 MHz, 0.22 MPa) of fluorescently labeled siRNA resulted in 97.9 ± 1.5% transfected cells, with siRNA localized homogenously in the cytoplasm directly after ultrasound exposure. UMTD of fluorescently labeled pDNA resulted in only 43.0 ± 4.2% transfected cells, with localization mainly in vesicular structures, co-localizing with endocytosis markers clathrin and caveolin. Delivery of siRNA against GAPDH (glyceraldehyde-3-phosphate dehydrogenase) effectively decreased protein levels to 24.3 ± 7.9% of non-treated controls (p < 0.01). In contrast, 24 h after delivery of pDNA encoding GAPDH, no increase in protein levels was detected. Transfection efficiency, verified with red fluorescently labeled pDNA encoding enhanced green fluorescent protein, revealed that of the transfected cells, only 2.0 ± 0.7% expressed the transgene. In conclusion, the difference in localization between siRNA and pDNA after UMTD is an important determinant of the effectiveness of these genetic-based technologies.

References

Jan 15, 2000·The Journal of Biological Chemistry·G L LukacsA S Verkman
Jun 17, 2004·Circulation·Jean Davignon, Peter Ganz
May 4, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sophie Mehier-HumbertRichard H Guy
Aug 6, 2005·Biochemical and Biophysical Research Communications·Manabu Kinoshita, Kullervo Hynynen
Nov 12, 2005·British Journal of Haematology·Charles T Esmon
Apr 25, 2006·American Journal of Physiology. Heart and Circulatory Physiology·L J M JuffermansO Kamp
Apr 25, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Michel Félétou, Paul M Vanhoutte
May 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Svitlana Y BerezhnaAshok A Deniz
Sep 15, 2006·Progress in Biophysics and Molecular Biology·E VanBavel
Dec 1, 2006·Preparative Biochemistry & Biotechnology·Tsuyoshi SakodaMitsumasa Ohyanagi
Nov 29, 2007·Journal of Drug Targeting·Bernadet D M MeijeringLeo E Deelman
Feb 2, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Roosmarijn E VandenbrouckeNiek N Sanders
Jul 10, 2008·The Journal of Gene Medicine·Maayan Duvshani-EshetMarcelle Machluf
Sep 23, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoichi NegishiYukihiko Aramaki
Oct 28, 2008·Ultrasound in Medicine & Biology·Vincenzo LionettiEugenio Picano
Nov 4, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kentaro OtaniNoritoshi Nagaya
Sep 22, 2009·Ultrasound in Medicine & Biology·Lynda J M JuffermansRené J P Musters
Oct 16, 2009·Ultrasound in Medicine & Biology·Joerg HauserManfred Hauser
May 10, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Linsey C PhillipsJohn A Hossack
Jul 2, 2011·The Journal of Gene Medicine·Daisy Maria Bentes PaulaSang Won Han
Aug 13, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Charles F CaskeyKatherine W Ferrara
Sep 6, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anthony DelalandeChantal Pichon
Jul 18, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Caitlin W BurkeRichard J Price

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Citations

Dec 3, 2014·Ultrasound in Medicine & Biology·Rick F J KwekkeboomRené J P Musters
Jul 23, 2016·Ultrasound in Medicine & Biology·Sandra NwokeohaRobin O Cleveland
Sep 18, 2016·Ultrasound in Medicine & Biology·Martynas MaciulevičiusSaulius Šatkauskas
May 28, 2016·Journal of the American College of Cardiology·Steven B Feinstein
Aug 22, 2018·Oncology Letters·Xitian LiangWen Cheng
Dec 13, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Eric K JuangMichalakis Averkiou
Apr 26, 2020·Ultrasound in Medicine & Biology·Antoine PressetNoboru Sasaki

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