Ultrasound-assisted emulsification microextraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry for the analysis of ibuprofen and its metabolites in human urine

Journal of Pharmaceutical and Biomedical Analysis
Sylwia Magiera, Şefika Gülmez

Abstract

In this study, a fast, simple and efficient method based on ultrasound-assisted emulsification-microextraction (USAEME) coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was successfully developed for the determination of ibuprofen (IBU) and its four metabolites (1-hydroxyibuprofen (1-HIBU), 2-hydroxyibuprofen (2-HIBU), 3-hydroxyibuprofen (3-HIBU), carboxyibuprofen (CIBU)) in human urine. For this purpose, the influence of the different parameters affecting the USAEME procedure was evaluated in order to optimize the efficiency of the process. The optimum conditions were found to be: 100μL of 1-octanol as extraction solvent, 2mL of urine sample, 15% (w/v) NaCl to control the ionic strength, ultrasonication for 10min; and centrifugation for 5min at 6500rpm. After sample preparation, chromatographic separation was achieved on a Zorbax Rapid Resolution High Definition (RRHD) SB-C18 column using the mobile phase consisting of 0.1% formic acid in water and acetonitrile in an elution gradient. Detection was performed in a triple quadrupole tandem mass spectrometer using the multiple reaction monitoring (MRM) mode and negative ionization. The proposed method showed satisfactory linearity o...Continue Reading

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Citations

Jan 13, 2016·Analytica Chimica Acta·Juan Antonio Ocaña-GonzálezMaría Ramos-Payán
Jul 18, 2018·Environmental Technology·Ricado SalgadoMaria T Barreto Crespo
Jun 12, 2019·Journal of Medical Microbiology·Samantha A WhitesideJeremy P Burton

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