Ultrasound-assisted extraction for the analysis of phenolic compounds in strawberries

Analytical and Bioanalytical Chemistry
M C Herrera, M D Luque de Castro

Abstract

A semiautomatic method based on application of ultrasounds has been developed to leach and hydrolyse phenolic compounds, such as naringin, rutin, naringenin, ellagic acid, quercetin and kaempferol, from strawberries. Two grams of lyophilized sample was placed into a sample cell and 5 mL of acetone containing hydrochloric acid was added. The cell was immersed in a water bath and sonicated for 30 s (duty cycle 0.8 s, output amplitude 50% of the nominal amplitude of the converter, applied power 100 W and with the probe placed 2 cm from the top surface of the extraction cell) for three times: each time 5 mL extractant displaced the previous extract. When the extraction was completed, the combined extracts were evaporated for 10 min, diluted to 10 mL with water adjusted to pH 8, and transferred to a cleanup-preconcentration manifold; here the analytes were retained in two in-series minicolumns packed with HR-P sorbent and then eluted with 4 mL methanol, and injected for individual separation-quantitation into a chromatograph-photodiode array detector assembly. Optimisation of the extraction was carried out using samples spiked with 4 mg kg(-1) of each analyte. Calibration curves using the standard addition in red strawberries typica...Continue Reading

Citations

May 4, 2013·Journal of Chromatography. a·M M Delgado-Povedano, M D Luque de Castro
Aug 14, 2013·Plant Physiology and Biochemistry : PPB·Walid Abu El-SoudHan Asard
Oct 21, 2014·Journal of Food Science and Technology·Alev Emine InceGulum Sumnu
Jan 22, 2014·Food Chemistry·Beatriz A Acosta-EstradaSergio O Serna-Saldívar
Feb 28, 2013·Food Chemistry·T Jerman Klen, B Mozetič Vodopivec
Mar 28, 2007·Analytica Chimica Acta·M D Luque de Castro, F Priego-Capote
Jan 31, 2006·Journal of Chromatography. a·R Japón-LujánM D Luque de Castro
Nov 26, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·H P Vasantha RupasingheGwendolyn M Huber
Oct 23, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin Dai, Russell J Mumper
Jan 4, 2017·Oxidative Medicine and Cellular Longevity·Aurelia Magdalena PisoschiGabriel Predoi
Jul 10, 2018·Food Science & Nutrition·Domenico CifáNataša Poklar Ulrih
Jul 21, 2015·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Sangeeta SaikiaCharu Lata Mahanta
Feb 25, 2021·Journal of Food Biochemistry·Fernando Guerrero-RomeroClaudia I Gamboa-Gómez
Sep 30, 2005·Journal of Agricultural and Food Chemistry·Zoi PapoutsiParaskevi Moutsatsou

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