Ultrasound-Assisted Extraction of Bioactive Compounds from Annatto Seeds, Evaluation of Their Antimicrobial and Antioxidant Activity, and Identification of Main Compounds by LC/ESI-MS Analysis

International Journal of Food Science
Julian Quintero QuirozJohn Jairo Rojas Camargo

Abstract

This study evaluated the antimicrobial activity (i.e., against Bacillus cereus and Staphylococcus aureus) and the antioxidant activity (i.e., ABTS, FRAP, and DPPH) of annatto seeds extract obtained by ultrasound-assisted extraction. A response surface design with three levels such as pH (2-11), solvent concentration (50-96 %), seed-to-solvent ratio (1:2-1:10), and treatment time (0-30 min) was employed to determine the optimal experimental conditions. Thus, a pH of 7.0, seed-to-solvent ratio of 1:7, and treatment time of 20 min were selected as optimal rendering an extract having a 0.62% of bixin, 3.81 mg gallic acid/mg equivalent of polyphenol compounds (ABTS 1035.7, FRAP 424.7, and DPPH 1161.5 μM trolox/L), and a minimal inhibitory concentration against Bacillus cereus and Staphylococcus aureus of 32 and 16 mg/L, respectively. Further, the main bioactive compounds identified by LC/ESI-MS were bixin and catechin, chlorogenic acid, chrysin, butein, hypolaetin, licochalcone A, and xanthohumol.

References

Dec 3, 2005·International Journal of Antimicrobial Agents·T P Tim Cushnie, Andrew J Lamb
Apr 30, 2010·Database : the Journal of Biological Databases and Curation·V NeveuA Scalbert
Nov 10, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Liang-Liang ZhangJia-Hong Chen
Oct 21, 2014·Journal of Food Science and Technology·Alev Emine InceGulum Sumnu
Oct 22, 2016·Food Chemistry·Fátima Rubio-SenentGuillermo Rodríguez-Gutiérrez

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