Ultrasound assisted gene and photodynamic synergistic therapy with multifunctional FOXA1-siRNA loaded porphyrin microbubbles for enhancing therapeutic efficacy for breast cancer

Biomaterials
Ranran ZhaoShumin Wang

Abstract

To improve the non-invasive therapeutic efficacy for ER positive breast cancer (ER+ BC), we fabricated a multifunctional FOXA1 loaded porphyrin microbubble to combine photodynamic therapy (PDT) and gene therapy of FOXA1 knockdown (KD) with ultrasound targeted microbubble destruction (UTMD) technology under the guidance of contrast enhanced ultrasound (CEUS). Cationic porphyrin microbubbles (CpMBs) were firstly fabricated from a porphyrin grafted lipid with two cationic amino groups (PGL-NH2) and fluorocarbon inert gas of C3F8. Porphyrin group in the CpMBs monolayer could be used as a photosensitizer for PDT, while amino groups could adsorb siRNA through electrostatic interaction for FOXA1 KD, which could inhibit the proliferation of estrogen-dependent ER+ BC. This system showed high photosensitizer and gene loading content. Moreover, CpMBs/siRNA can be converted into nanoparticles with low-frequency pulsed ultrasound (LFUS) exposure, which increase the transfection efficiency of siRNA (∼4 fold) and the porphyrin uptake (∼8 fold) in MCF-7 (a human breast cancer cell line, ER+) by sonoporation effect. In vivo, UTMD was performed under the guidance of CEUS, and the fluorescence intensity of CpMBs/siRNA at the tumour site reached a...Continue Reading

Citations

Apr 2, 2019·Journal of Zhejiang University. Science. B·Li-Ying Wang, Shu-Sen Zheng
Dec 14, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Sadaf HameedZhifei Dai
Jun 23, 2020·Experimental Biology and Medicine·Zhiyong ShenWeixing Qu
Feb 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Elżbieta OstańskaDorota Bartusik-Aebisher
Jan 1, 2021·Frontiers in Oncology·Mariela Montaño-SamaniegoMiguel Ibáñez-Hernández
Mar 9, 2021·Science China. Chemistry·Chen ZhangZhe Liu
Jul 27, 2021·Ultrasound in Medicine & Biology·Lixia Mei, Zhen Zhang
Aug 25, 2021·ACS Biomaterials Science & Engineering·Kun LiYuanyu Huang
Sep 11, 2021·Journal of Biomaterials Science. Polymer Edition·In Jae ChungCheol-Hee Ahn

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