Ultrasound bath-assisted enzymatic hydrolysis procedures as sample pretreatment for the multielement determination in mussels by inductively coupled plasma atomic emission spectrometry

Analytical Chemistry
Carlos Peña-FarfalIda de Gregori-Henríquez

Abstract

Ultrasound energy has been applied to speed up enzymatic hydrolysis processes of mussel tissue in order to determine trace and ultratrace elements (As, Al, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn). The element releases, by action of three proteases (pepsin, pancreatin, trypsin), lipase, and alpha-amylase, have been evaluated by inductively coupled plasma atomic emission spectrometry. Different variables such as pH, sonication temperature, ionic strength, hydrolysis time, ultrasound frequency, extracting volume, and enzyme mass were simultaneously studied by applying an experimental design approach (Plackett-Burman design and central composite design). Results showed that the hydrolysis time was statistically nonsignificant (confidence interval of 95%) for most of the elements and enzymes, meaning that the hydrolysis procedure can be finished within a 30-60-min range. These hydrolysis times are far shorter than those obtained when using thermostatic cameras, between 12 and 24 h. Statistically significant factors were the ultrasound frequency (the highest metals releasing at high-ultrasound frequency), pH, sonication temperature, and ionic strength. All metals can be extracted using the same operating conditions (pH of 1.0 and sodium chlo...Continue Reading

References

Apr 10, 2002·Analytical and Bioanalytical Chemistry·Christian WolfPeter Brätter

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Citations

Mar 31, 2005·Analytical and Bioanalytical Chemistry·Christine ScriverH Birnboim
Sep 28, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M D Luque de CastroA Peralbo-Molina
Dec 17, 2009·Journal of Chromatography. a·José L TadeoAna I García-Valcárcel
Apr 12, 2013·Journal of the Science of Food and Agriculture·Zeynep IlbayS İsmail Kırbaşlar
Aug 29, 2007·Analytica Chimica Acta·Jorge Moreda-PiñeiroPilar Bermejo-Barrera
Nov 4, 2006·Journal of Biochemical and Biophysical Methods·F Priego-Capote, Luque de Castro
Oct 12, 2013·Journal of Chromatography. a·Martha Míguez-FramilAntonio Moreda-Piñeiro
Nov 16, 2020·Journal of Food Science·Zijian LiangPangzhen Zhang
Feb 3, 2007·Analytical Chemistry·Antonio Moreda-PiñeiroDarío Prada-Rodríguez
Jan 31, 2020·Journal of Agricultural and Food Chemistry·Caleb LuvongaSang Bok Lee

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