PMID: 9192358May 1, 1997Paper

Ultrasound-guided follicle aspiration and IVF in dairy cows treated with FSH after removal of the dominant follicle at different stages of the estrous cycle [corrected

The Journal of Veterinary Medical Science
M OoeT Suzuki

Abstract

Recently, transvaginal ultrasound-guided follicle aspiration technology has been found to be of great value for in vitro fertilization (IVF) programs, even though the oocyte recovery rate and cleavage rate of transferrable embryos were low. In this study, we investigated the effect of the removal of the dominant follicle at different stages of the estrous cycle on the ovarian response of donor cows. Four experiments (EXPs) were devised. In EXP 1, 3 cows received 20 mg FSH on Day 1, ovulation occurred on Day 0, and on Day 3 follicles were aspirated. In EXP 2, the dominant follicle of the first wave was removed on Day 6 from 3 cows which received 20 mg FSH on Day 7 and on Day 9 follicles were aspirated. In EXP 3, 2 pregnant cows received 20 mg FSH on 70 d of pregnancy and 48 hr later follicles were aspirated a total of 5 times at 5-day intervals. In EXP 4, after ovulation on Day 0, 9 cows received 20 mg FSH on Days 8 to 14 of the estrous cycle and 48 hr after the last injection, follicles were aspirated once. The respective mean +/- SD numbers of aspirated follicles and recovered oocytes were higher (p < 0.01) in EXP 1 (13.4 +/- 1.7 and 8.7 +/- 2.3), EXP 2 (12.1 +/- 1.4 and 7.7 +/- 1.7) and EXP 3 (10.7 +/- 2.1 and 7.0 +/- 2.2) th...Continue Reading

References

Mar 1, 1975·Biology of Reproduction·B G Brackett, G Oliphant
Jan 1, 1992·Journal of Reproduction and Fertility·G P AdamsO J Ginther
Jan 1, 1991·Journal of Reproduction and Fertility·L A GuilbaultP Matton
Mar 1, 1991·Journal of Reproduction and Fertility·J C KoO J Ginther
Jan 1, 1991·Journal of Dairy Science·C Taylor, R Rajamahendran
Sep 1, 1989·Journal of Reproduction and Fertility·O J GintherJ P Kastelic
Aug 1, 1989·Biology of Reproduction·O J GintherJ P Kastelic
Jul 1, 1988·Journal of Reproduction and Fertility·J D SavioJ F Roche

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