Ultrasound-guided intrauterine injection of lipopolysaccharide as a novel model of preterm birth in the mouse

The American Journal of Pathology
Sara F RinaldiSarah Stock

Abstract

Mouse models are used to study mechanisms that link intrauterine infection and preterm birth (PTB). To mimic intrauterine infection, lipopolysaccharide (LPS) is commonly injected into the uterus via minilaparotomy, which is invasive, and can cause PTB in control animals. We hypothesized that less-invasive ultrasound-guided intrauterine LPS injection or intravaginal LPS administration could induce PTB by stimulating an inflammatory response of the uteroplacental tissues, while minimizing PTB in control animals. On day 17 of gestation mice received LPS intravaginally (10 to 240 μg; n = 3 to 8) or into the uterus (20 μg) under ultrasound guidance (n = 7) or via laparotomy (n = 7). Control animals received phosphate-buffered saline (PBS; n = 5 to 7). Intrauterine administration of LPS, both under ultrasound guidance and via laparotomy, induced delivery earlier than in PBS control groups (P < 0.01). Intravaginal LPS administration did not stimulate PTB. Quantitative real-time PCR and immunohistochemistry of tissues harvested 6 hours after treatment confirmed that ultrasound-guided LPS administration induced a localized inflammatory response. Ultrasound-guided intrauterine LPS injection reliably induces PTB in the mouse and mimics th...Continue Reading

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Citations

Feb 1, 2017·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Nardhy Gomez-LopezSonia S Hassan
Dec 23, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Sara R van BoeckelSarah J Stock
Oct 13, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ashley K BoyleJane E Norman
May 1, 2020·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Jaroslav StranikIvana Musilova
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Nov 22, 2017·Biology of Reproduction·Shanmugasundaram NallasamyMala Mahendroo
Feb 11, 2020·Frontiers in Immunology·Steven M Yellon
Apr 17, 2019·Tissue Engineering and Regenerative Medicine·Yoon Young KimSeung-Yup Ku
May 16, 2019·Scientific Reports·Sara R van BoeckelSarah J Stock
May 7, 2020·Frontiers in Immunology·Monica CappellettiSuhas G Kallapur
Nov 20, 2020·JCI Insight·Monica CappellettiSenad Divanovic
Dec 15, 2019·The American Journal of Pathology·Hannah C ZierdenLaura M Ensign

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