Ultrasound imaging of oxidative stress in vivo with chemically-generated gas microbubbles.

Annals of Biomedical Engineering
John Kangchun PerngNiren Murthy

Abstract

Ultrasound contrast agents (UCAs) have tremendous potential for in vivo molecular imaging because of their high sensitivity. However, the diagnostic potential of UCAs has been difficult to exploit because current UCAs are based on pre-formed microbubbles, which can only detect cell surface receptors. Here, we demonstrate that chemical reactions that generate gas forming molecules can be used to perform molecular imaging by ultrasound in vivo. This new approach was demonstrated by imaging reactive oxygen species in vivo with allylhydrazine, a liquid compound that is converted into nitrogen and propylene gas after reacting with radical oxidants. We demonstrate that allylhydrazine encapsulated within liposomes can detect a 10 micromolar concentration of radical oxidants by ultrasound, and can image oxidative stress in mice, induced by lipopolysaccharide, using a clinical ultrasound system. We anticipate numerous applications of chemically-generated microbubbles for molecular imaging by ultrasound, given ultrasound's ability to detect small increments above the gas saturation limit, its spatial resolution and widespread clinical use.

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Citations

Jan 8, 2013·Trends in Cardiovascular Medicine·Jesper HjortnaesElena Aikawa
Aug 21, 2013·Biomaterials·Emilia S OlsonRobert F Mattrey
Apr 19, 2015·European Heart Journal·Keyvan Karimi GalougahiGemma A Figtree
May 4, 2016·Antioxidants & Redox Signaling·Giuseppe MaulucciMarco De Spirito
May 18, 2016·British Journal of Pharmacology·Andreas DaiberThomas Münzel
Jan 13, 2017·Advanced Healthcare Materials·Emilia S OlsonRobert Mattrey
Jul 12, 2017·Journal of Drug Targeting·Sungmun LeeYeu-Chun Kim
Jul 24, 2014·Organic & Biomolecular Chemistry·Mina Barzegar Amiri OliaMichelle K Taylor
Jan 2, 2021·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Eli M EspinozaNiren Murthy

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