Ultrasound mediated intravesical transfection enhanced by treatment with lidocaine or heat

The Journal of Urology
Ryohei OgawaTakashi Kondo

Abstract

We have previously reported that cell membrane modification by lidocaine or heat can enhance ultrasound mediated transfection (USMT) on PC-3 cells in vitro. In the current study we investigated whether such enhancement could be observed using the T24 human bladder cancer cell line in vitro along with PC-3 in vivo. For in vitro transfection T24 cells were sonicated with 1 MHz ultrasound at 3.6 W/cm (ISATA) for 20 seconds. For in vivo transfection T24 or PC-3 cells in the bladder were transabdominally sonicated with 1 MHz ultrasound at 0.78 W/cm (ISATA) for 60 seconds. Transfection efficiency was evaluated by the luciferase assay standardized with protein contents of the samples. Lidocaine or heat treatment of T24 cells during sonication enhanced luciferase expression significantly. Results indicated that enhancements could be achieved in a different cell line, although to lesser degrees than with PC-3 cells. In addition, membrane fluidity facilitation and cell viability after sonication were also different, presumably influenced by the different structures and/or compositions of the cell membranes. PC-3 and T24 cells were successfully transfected in the bladder. In addition, USMT enhancements were also observed in the 2 cell lin...Continue Reading

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Citations

Aug 8, 2006·Molecular Pharmaceutics·Pradeep TyagiLeaf Huang
Apr 6, 2007·The AAPS Journal·Xiang GaoDexi Liu
Oct 17, 2009·The AAPS Journal·Mohammed S Al-Dosari, Xiang Gao
Mar 1, 2009·Journal of Medical Ultrasonics·Kazuki YamaguchiKatsuro Tachibana
Jan 1, 2010·Journal of Medical Ultrasonics·Loreto B FerilYoshimi Harada
May 25, 2012·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Loreto B Feril, Katsuro Tachibana
Aug 24, 2006·Cancer Science·Loreto B FerilTakashi Kondo
Jul 5, 2011·Biochemical and Biophysical Research Communications·Kazuki YamaguchiJuichiro Nakayama
May 23, 2007·BioTechniques·Ryohei OgawaKazutaka Yamamoto
May 20, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Farzan SiddiquiJae Ho Kim

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