Ultrasound-mediated nanobubble destruction (UMND) facilitates the delivery of A10-3.2 aptamer targeted and siRNA-loaded cationic nanobubbles for therapy of prostate cancer

Drug Delivery
Meng WuJie Tang

Abstract

The Forkhead box M1 (FoxM1) transcription factor is an important anti-tumor target. A novel targeted ultrasound (US)-sensitive nanobubble that is likely to make use of the physical energy of US exposure for the improvement of delivery efficacy to target tumors and specifically silence FoxM1 expression appears as among the most potential nanocarriers in respect of drug delivery. In this study, we synthesized a promising anti-tumor targeted FoxM1 siRNA-loaded cationic nanobubbles (CNBs) conjugated with an A10-3.2 aptamer (siFoxM1-Apt-CNBs), which demonstrate high specificity when binding to prostate-specific membrane antigen (PSMA) positive LNCaP cells. Uniform nanoscaled siFoxM1-Apt-CNBs were developed using a thin-film hydration sonication, carbodiimide chemistry approaches, and electrostatic adsorption methods. Fluorescence imaging as well as flow cytometry evidenced the fact that the siFoxM1-Apt-CNBs were productively developed and that they specifically bound to PSMA-positive LNCaP cells. siFoxM1-Apt-CNBs combined with ultrasound-mediated nanobubble destruction (UMND) significantly improved transfection efficiency, cell apoptosis, and cell cycle arrest in vitro while reducing FoxM1 expression. In vivo xenografts tumors in nu...Continue Reading

References

Jul 4, 1998·Ultrasound in Medicine & Biology·W J GreenleafJ F Greenleaf
May 31, 2003·Somatic Cell and Molecular Genetics·Douglas L MillerJames E Greenleaf
Dec 31, 2003·Ultrasound in Medicine & Biology·Jonathan P ChristiansenJonathan R Lindner
Nov 3, 2004·Cancer Research·Omid C FarokhzadRobert Langer
Feb 1, 2005·Journal of the American College of Cardiology·Raffi BekeredjianRalph V Shohet
Jul 12, 2007·Journal of the National Cancer Institute·Natalya RapoportAnne Kennedy
Oct 13, 2007·Biochemistry·Zhen HuangLi Niu
May 9, 2009·Advanced Drug Delivery Reviews·Yu-Kyoung Oh, Tae Gwan Park
Jul 2, 2009·Methods in Molecular Biology·Sunjoo JeongMee Young Kim
Sep 29, 2009·International Journal of Pharmaceutics·Ye WangYuhong Xu
Apr 20, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yuanyuan QiuJuan Tu
Jul 6, 2010·Cancer Letters·Nikolitsa Nomikou, Anthony P McHale
Aug 31, 2010·Ultrasound in Medicine & Biology·Jose L TlaxcaJoshua J Rychak
Jan 29, 2011·Cell Cycle·Tanya V KalinVladimir V Kalinichenko
Dec 7, 2011·Biomaterials·Chung-Hsin WangChih-Kuang Yeh
Mar 29, 2014·International Journal of Molecular Medicine·Yiru WangJie Tang
Aug 31, 2016·International Journal of Nanomedicine·Xiaozhou FanKejing Fang

❮ Previous
Next ❯

Citations

Aug 3, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Padmanaban SivakumarMin Suk Shim
May 6, 2019·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Dongyong ZhuMeng Wu
Aug 23, 2019·Current Drug Delivery·Pritish Kumar PandaSanjay K Jain
Jun 20, 2019·Frontiers in Pharmacology·Jiaqi ZhangMingxing Xie
Feb 23, 2020·Frontiers in Pharmacology·Xiaowen CaiChuanshan Xu
Jan 1, 2020·Pharmaceutics·Ana Paula Dinis Ano BomSotiris Missailidis
Apr 23, 2020·International Journal of Molecular Sciences·Zhaoying Fu, Jim Xiang
Sep 3, 2020·Nanomaterials·Sarkar Siddique, James C L Chow
Dec 4, 2020·International Journal of Molecular Sciences·Zhaoying Fu, Jim Xiang
Jun 30, 2019·Heliyon·Akiko WatanabeKatsuro Tachibana
Jul 8, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Reza MohammadinejadAli Zarrabi
May 12, 2021·Chemical Society Reviews·Ranhua XiongKevin Braeckmans
May 30, 2021·Prostate Cancer and Prostatic Diseases·Fujin WangMei Lin
Jun 14, 2021·Advances in Colloid and Interface Science·Marie Pierre Krafft, Jean G Riess
Aug 31, 2021·ACS Biomaterials Science & Engineering·Kahkashan BansalHimanshu Shekhar

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
xenografts
electron microscopy
dynamic light scattering
flow cytometry
electrophoresis
xenograft
PCR
Protein Assay
fluorescence

Software Mentioned

SPSS
DFY
SonoVue

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis