Ultrasound microbubble-carried PNA targeting to c-myc mRNA inhibits the proliferation of rabbit iliac arterious smooth muscle cells and intimal hyperplasia

Drug Delivery
Xia HeYue-Xuan Chen

Abstract

To elucidate the transfected effect of albumin ultrasound microbubbles carrying peptide nucleic acids (PNAs) against c-myc gene to the vascular walls and their effect on the intimal proliferation induced by vascular denudation. A rabbit iliac artery intimal proliferation model was constructed and PNA against c-myc mRNA was designed and synthesized and was added to albumin solution before ultrasound microbubbles were prepared and encapsulated in matrix of albumin. The ultrasound microbubbles carrying PNA were transfected to intima under ultrasound exposure. The transfected effect was identified by a histochemical method and the expression of c-myc was detected by in situ hybridization. The proliferation of intimal smooth muscle cells was estimated by the expression of proliferative cell nuclear antigen (PCNA) of them. The intimal area and thickness were judged morphologically for intimal hyperplasia. The ultrasound microbubbles with PNA were successfully prepared and c-myc PNA was transfected to vascular intimal cells. The expression of c-myc and PCNA by intimal vascular smooth muscle cells (vSMCs) was inhibited significantly and the intimal thickness and area were reduced remarkably. Transfection of c-myc PNA could inhibit prol...Continue Reading

References

Feb 15, 2001·Gene Therapy·A LawrieC M Newman
Feb 10, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Ashwani Khanna
Mar 25, 2009·British Journal of Pharmacology·Frederic HeitzGilles Divita
Aug 7, 2010·Current Pharmaceutical Design·Peter E Nielsen
May 17, 2011·The Journal of Surgical Research·Jun WangHua Jing
Jul 19, 2011·Research in Veterinary Science·Dong-Jun AnBong-Kyun Park
Aug 29, 2012·Artificial DNA, PNA & XNA·Davide BonifaziArnaud Delforge
Feb 6, 2013·Theranostics·Shashank R Sirsi, Mark A Borden
Mar 19, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·K Heath Martin, Paul A Dayton
Jul 17, 2013·Journal of Cardiovascular Translational Research·Richard G LeeRosanne M Crooke
Jan 1, 2012·Pharmaceuticals·Alfredo Erazo-OliverasJean-Philippe Pellois
Dec 4, 2013·Methods in Molecular Biology·Henrik StenderJames Coull
Jan 11, 2014·Expert Opinion on Therapeutic Patents·Roberto Gambari
Mar 22, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ryan R SunCarol H Miao
May 7, 2014·Expert Opinion on Therapeutic Patents·Santiago GrijalvoRamon Eritja

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Citations

Feb 23, 2020·Frontiers in Pharmacology·Xiaowen CaiChuanshan Xu
Nov 20, 2020·European Journal of Nuclear Medicine and Molecular Imaging·Kai ZhangHong Zhang

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