Ultrasound-modulated fluorescence based on donor-acceptor-labeled microbubbles

Journal of Biomedical Optics
Yuan LiuBaohong Yuan

Abstract

A fluorescence resonance energy transfer (FRET)-based microbubble contrast agent system was designed to experimentally demonstrate the concept of ultrasound-modulated fluorescence (UMF). Microbubbles were simultaneously labeled with donor and acceptor fluorophores on the surface to minimize self-quenching and maximize FRET. In response to ultrasound, the quenching efficiency was greatly modulated by changing the distance between the donor and acceptor molecules through microbubble size oscillations. Both donors and acceptors exhibited UMF on individual microbubbles. The UMF strength of the donor was more significant compared to that of the acceptor. Furthermore, the UMF of the donor was observed from a microbubble solution in a turbid media. This study exploits the feasibility of donor–acceptor labeled microbubbles as UMF contrast agents.

References

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Aug 12, 2014·PloS One·Christopher W JarrettJohn C Gore
Aug 28, 2014·Journal of Biomedical Optics·Bahar SaremiBaohong Yuan

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Citations

Jul 29, 2016·PloS One·Qimei ZhangMelissa L Mather
May 14, 2019·Frontiers in Bioengineering and Biotechnology·Yanbo Pei, Ming-Yuan Wei

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