PMID: 6966646May 1, 1980Paper

Ultrastruct and tartrate-resistant acid phosphatase localization in a T-cell hairy-cell leukemia cell line

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
S G QuanD W Golde

Abstract

Hairy-cell leukemia is characterized clinically in splenomegaly and pancytopenia and pathologically by the proliferation in hematopoietic tissue of cells containing the tartrate-resistant isozyme 5 of acid phosphatase. We have described a patient with a T-lymphocyte variant of this disease. A permanent cell line obtained from the spleen of this patient has the biological and enzymatic characteristics of the fresh leukemic cells. We have used this line to study the surface morphology, ultrastructure, and ultrastructural localization of acid phosphatase in defined T-lymphoid hairy cells. The surface of the cells of the permanent line was smooth but many hair-like projections appeared after exposure to phytohemagglutinin (PHA). There was little acid phosphatase reaction produce visualized when beta-glycerophosphate was used as a substrate. With sodium haphthol AS-BI phosphoric acid heavy deposits were seen in the perinuclear membrane, mitochondria, and rough endoplasmic reticulum. Exposure to PHA and pokeweed mitogen resulted in increased reaction product, suggesting increased enzyme synthesis. Tartrate-resistant acid phosphatase was localized in the same organelles.

Citations

Jul 1, 1992·Calcified Tissue International·J I AlvarezS L Teitelbaum
Jan 1, 1983·Acta Neuropathologica·M Elleder
Jan 1, 1988·Immunology Letters·L F UmekitaI Mota
Feb 1, 1985·European Journal of Cancer & Clinical Oncology·R E MerchantS Arrenbrecht
Nov 1, 1988·The Journal of protozoology·N K MukhopadhyayR H Glew
Feb 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S H AllenR M Roberts
Jan 1, 1985·Microbiology and Immunology·Y TanakaY Hinuma
Jan 1, 1985·The British Journal of Ophthalmology·P N Dilly, I A Mackie
Nov 1, 1995·Ultrastructural Pathology·B P EydenS S Banerjee

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