Ultrastructural alterations of frozen-thawed Asian elephant (Elephas maximus) spermatozoa

International Journal of Andrology
M Sa-ArdritAnuchai Pinyopummin

Abstract

Intact plasma and acrosome membranes and functional mitochondria following cryopreservation are important attributes for the fertilizing ability of spermatozoa. In the present study, functional and ultrastructural changes of Asian elephant spermatozoa after cryopreservation either in TEST + glycerol or HEPT + dimethyl sulphoxide (DMSO) were evaluated by fluorescent techniques and electron microscopy. Sperm frozen in TEST + glycerol had higher proportion of sperm with intact plasma (49.1 +/- 9.2% vs. 30.9 +/- 3.9%) and acrosomal (53.7 +/- 4.9% vs. 35.8 +/- 6.1%) membranes, as well as active mitochondria (57.0 +/- 7.2% vs. 42.0 +/- 5.0%) than those cryopreserved in HEPT + DMSO. The results obtained from electron microscopy were similar to those obtained by fluorescence microscopy. The percentage of normal spermatozoa was higher when spermatozoa were frozen in TEST + glycerol than those frozen in HEPT + DMSO (31.8 +/- 5.6 vs. 28.5 +/- 6.4). The ultrastructural alterations revealed by transmission electron microscopy could be classified as (i) distension of plasma membrane, while the acrosome was swollen; (ii) disruption or loss of plasma membrane, while acrosome was swollen with distended outer acrosomal membrane; (iii) disruption...Continue Reading

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Citations

Sep 7, 2012·Reproduction, Fertility, and Development·P ImratT A E Stout
Mar 19, 2008·Reproductive Biology and Endocrinology : RB&E·Nikorn ThongtipAnuchai Pinyopummin
Jul 21, 2009·Reproductive Biology and Endocrinology : RB&E·Nikorn ThongtipKulnasan Saikhun
Mar 21, 2009·Theriogenology·J SaragustyA Arav
Dec 9, 2008·Animal Reproduction Science·Joseph SaragustyThomas B Hildebrandt
Dec 26, 2008·Animal Reproduction Science·Joseph SaragustyAmir Arav
Dec 30, 2008·Animal Reproduction Science·Joseph SaragustyRobert Hermes
Apr 28, 2007·Cryobiology·Rhett V McCleanStephen D Johnston
Jul 14, 2009·Biotechnology and Bioengineering·Joseph SaragustyAmir Arav
Oct 9, 2018·Microscopy Research and Technique·Luana Grasiele Pereira BezerraAlexandre Rodrigues Silva
Oct 20, 2018·Anatomia, histologia, embryologia·Lucia OlexikovaPeter Chrenek
Dec 22, 2011·The Journal of Reproduction and Development·Shlomo OrgalZvi Roth
Aug 21, 2020·Biopreservation and Biobanking·Nazan KeskinSukru Dursun

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