PMID: 9446831Apr 4, 1998Paper

Ultrastructural analysis of human epidermal CD44 reveals preferential distribution on plasma membrane domains facing the hyaluronan-rich matrix pouches

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
A L TuhkanenR Tammi

Abstract

We used immunogold staining and stereology to examine the ultrastructural localization and to estimate the relative content of CD44 in different strata and cell types of normal human epidermis. We found that CD44 existed almost exclusively on the plasma membranes; only rare labeling occurred on vesicular structures within the cytoplasm. Quantitation of the immunogold particles indicated that the labeling density of melanocytes corresponded to that of basal keratinocytes, and Langerhans cells displayed a labeling density of approximately 10% that of the surrounding spinous cells. Among keratinocyte strata, the highest labeling density occurred on spinous cells, suggesting upregulation of CD44 after detachment from the basement membrane. The plasma membrane distribution of CD44 was compartmentalized, with little signal on cell-cell and cell-substratum contact sites such as desmosomes, the plasma membrane domain facing the basement membrane, and the close apposition of terminally differentiating granular cells. In contrast, CD44 was abundant on plasma membrane domains facing an open intercellular space, rich in hyaluronan. This distribution is in line with a role of CD44 as a hyaluronan receptor, important in the maintenance of th...Continue Reading

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Citations

Nov 7, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J W StevensJ A Maynard
Nov 11, 2011·Histochemistry and Cell Biology·Sanna Pasonen-SeppänenRaija Tammi
Jan 22, 2003·The British Journal of Dermatology·S KarvinenR Tammi
May 7, 1999·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·W Y Chen, G Abatangelo
Oct 1, 2005·American Journal of Respiratory Cell and Molecular Biology·Maria E MonzonRosanna M Forteza
Dec 2, 2004·Archives of Histology and Cytology·Tadashi YasuiWilfried Meyer
Sep 9, 1999·In Vitro Cellular & Developmental Biology. Animal·J ZhouL M Milstone
Jul 28, 2015·The Journal of Investigative Dermatology·Jérémy MalaisseYves Poumay
Mar 25, 2014·The Journal of Investigative Dermatology·Jérémy MalaisseYves Poumay
Jun 9, 2000·The Journal of Investigative Dermatology·S SakaiS Inoue
Sep 18, 2009·Experimental Dermatology·Søren KampTomas Norman Dam
Nov 24, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A L TuhkanenR H Tammi
Feb 7, 2018·The British Journal of Dermatology·L HämäläinenR H Tammi
Mar 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Koert P DingemansSteven T Pals
Jul 6, 2020·Matrix Biology : Journal of the International Society for Matrix Biology·Anna MichopoulouPatricia Rousselle
Mar 24, 2021·The American Journal of Pathology·Shunsuke IriyamaSatoshi Amano
Apr 14, 2021·Scientific Reports·Delphine Del MarmolBruno Flamion
Aug 6, 2021·Frontiers in Genetics·Adam AbiedJoram M Mwacharo

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