Ultrastructural analysis of primary human urethral epithelial cell cultures infected with Neisseria gonorrhoeae.

Infection and Immunity
H A HarveyM A Apicella

Abstract

In men with gonococcal urethritis, the urethral epithelial cell is a site of infection. To study the pathogenesis of gonorrhea in this cell type, we have developed a method to culture primary human urethral epithelial cells obtained at the time of urologic surgery. Fluorescent analysis demonstrated that 100% of the cells stained for keratin. Microscopic analyses indicated that these epithelial cells arrayed in a pattern similar to that seen in urethral epithelium. Using immunoelectron and confocal microscopy, we compared the infection process seen in primary cells with events occurring during natural infection of the same cell type in men with gonococcal urethritis. Immunoelectron microscopy studies of cells infected with Neisseria gonorrhoeae 1291 Opa+ P+ showed adherence of organisms to the epithelial cell membrane, pedestal formation with evidence of intimate association between the gonococcal and the epithelial cell membranes, and intracellular gonococci present in vacuoles. Confocal studies of primary urethral epithelial cells showed actin polymerization upon infection. Polyclonal antibodies to the asialoglycoprotein receptor (ASGP-R) demonstrated the presence of this receptor on infected cells in the primary urethral cell...Continue Reading

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Citations

Oct 19, 2004·Clinical Microbiology Reviews·Jennifer L Edwards, Michael A Apicella
Mar 24, 2005·Infection and Immunity·L L GreinerM A Apicella
Jan 26, 2016·Critical Reviews in Microbiology·Jennifer L EdwardsKate L Seib
Feb 13, 2001·Journal of Bacteriology·C D LongH S Seifert
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A J Merz, M So
Mar 21, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Louise StrömbeckInger Mattsby-Baltzer
Apr 2, 2005·Antonie van Leeuwenhoek·Liuh Ling GohTiow Suan Sim
Apr 30, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wen XuZheng-Feng Yin
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Ana Clara MendesDiana Bahia
Dec 14, 2018·Frontiers in Immunology·Jonathan D Lenz, Joseph P Dillard
Jan 17, 2019·The Journal of Antimicrobial Chemotherapy·Fabian Yuh Shiong KongJane S Hocking
Feb 27, 1999·Current Opinion in Microbiology·M NaumannT F Meyer
Aug 18, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Ursula TheuretzbacherEmilie Alirol

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