PMID: 2118560Oct 1, 1990Paper

Ultrastructural and immunocytochemical study of the uptake and distribution of bacterial lipopolysaccharide in human monocytes

Journal of Leukocyte Biology
Y H KangC H Lee

Abstract

Interaction of bacterial lipopolysaccharide (LPS) with monocytes stimulates production of a variety of mediators that are involved in the pathogenesis of septic shock and wound repair. We report here the mechanisms of LPS uptake and intracellular distribution of LPS in human monocytes. Ficoll-Hypaque-purified peripheral mononuclear cells (PBMC) were exposed to LPS from rough Escherichia coli (J5) or to biotin-conjugated LPS (biotin-LPS) from smooth E. coli (0111:B4), or to fluorescein isothiocyanate-conjugated LPS of E. coli (055:B5) at 37 degrees C for various times and processed for electron microscopy, immunocytochemistry, and flow cytometry. Monocytes were identified by the presence of numerous cytoplasmic peroxidase-positive granules or by monoclonal antibodies against monocyte. LPS micelles were identified by their specific bilayer structure, staining of horseradish peroxidase reaction product, or colloidal gold using biotin-LPS or a monoclonal antibody to LPS. Binding of LPS to cell surface was observed 5 min after incubation with LPS. Intracellular localization of LPS micelles was found 30 min following exposure to LPS. Prolonged incubation with LPS increased intracellular LPS. Intracellularly, LPS micelles were found i...Continue Reading

Citations

Aug 1, 1998·The Journal of Biological Chemistry·C PoussinJ Pugin
Aug 17, 1999·The Journal of Experimental Medicine·N Thieblemont, S D Wright
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G D KutuzovaN Qureshi
Apr 7, 2010·Immunopharmacology and Immunotoxicology·Concetta SaponaroMaria Antonietta Panaro
Apr 7, 1993·Molecular and Cellular Biochemistry·C RiscoJ L Carrascosa
Apr 28, 2006·Veterinary Research·Sarabjeet Singh SuriBaljit Singh
Apr 13, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Zhiqing XingRichard A Smith
Nov 22, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Garrett W HillMatthew R Kuennen
Sep 24, 2011·Mediators of Inflammation·Elias Leiva-SalcedoClaudio Acuña-Castillo
Dec 1, 1993·Infection and Immunity·M WuorelaK Granfors

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