Ultrastructural and molecular characterization of endosymbionts of the reed beetle genus Macroplea (Chrysomelidae, Donaciinae), and proposal of "Candidatus Macropleicola appendiculatae" and "Candidatus Macropleicola muticae"

Canadian Journal of Microbiology
Gregor KölschBo Vest Pedersen

Abstract

Intracellular bacterial symbionts are known from various insect groups, particularly from those feeding on unbalanced diets, where the bacteria provide essential nutrients to the host. In the case of reed beetles (Coleoptera: Chrysomelidae, Donaciinae), however, the endosymbionts appear to be associated with specialized "glands" that secrete a material used for the beetles' unusual water-tight cocoon. These glands were discovered over a century ago, but the bacteria they contain have yet to be characterized and placed in a phylogenetic context. Here, we describe the ultrastructure of two endosymbiotic species ("Candidatus Macropleicola appendiculatae" and "Candidatus Macropleicola muticae") that reside in cells of the Malpighian tubules of the reed beetle species Macroplea appendiculata and Macroplea mutica, respectively. Fluorescent in situ hybridization using oligonucleotides targeting the 16S rRNA gene specific to Macroplea symbionts verified the localization of the symbionts in these organs. Phylogenetic analysis of 16S rRNA placed "Candidatus Macropleicola" in a clade of typically endosymbiotic Enterobacteriaceae (gamma-proteobacteria). Finally, we discuss the evidence available for the hypothesis that the beetle larvae us...Continue Reading

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Citations

May 23, 2013·FEMS Microbiology Reviews·Philipp Engel, Nancy A Moran
May 14, 2016·Planta·Beata Wielkopolan, Aleksandra Obrępalska-Stęplowska
Dec 8, 2016·International Journal of Systematic and Evolutionary Microbiology·Aharon Oren
Dec 3, 2016·International Journal of Systematic and Evolutionary Microbiology·Aharon Oren
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Tobin J Hammer, Nancy A Moran
Jul 1, 2020·International Journal of Systematic and Evolutionary Microbiology·Aharon OrenMartha E Trujillo
Oct 5, 2021·Annual Review of Entomology·Hassan Salem, Martin Kaltenpoth

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