PMID: 7084572Jan 1, 1982Paper

Ultrastructural aspects of erythropoietic differentiation in long-term bone marrow culture

Differentiation; Research in Biological Diversity
T D Allen, T M Dexter

Abstract

Long-term liquid cultures of mouse bone marrow produce stem cell (CFU-S) and differentiated granulocytes for many months. Addition of AMS (anaemic mouse serum) to the cultures almost entirely eliminates the granulopoietic activity and stimulates erythropoiesis, with full erythroid maturation and the production of adult haemoglobin. Ultrastructural analysis of in situ fixed material reveals the cell shape and surface morphology of the erythroid maturation series, and the generation of erythroblastic islands in vitro. Each erythroblastic island consists of one or more synchronously maturing cohorts of erythroid cells undergoing four or five divisions between proerythroblast and normoblast. Each island is centered on a macrophage, which interacts with the developing erythroid population in several ways. Expelled nuclei are phagocytosed by te macrophage, which also has large areas of closely apposed membrane with the erythroid cells, gap junctions, and possible reciprocal vesicular activity. Changes in the adherent layer (stromal cells) also occur with the transition from granulopoiesis to erythropoiesis. There is a reduction in the endothelial cell cover, and mobilisation of lipid from the granulopoietic associated apidocytes.

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Oct 7, 1976·Differentiation; Research in Biological Diversity·T D Allen, T M Dexter
May 1, 1977·British Journal of Haematology·D Hemmaplardh, E H Morgan
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J R StephensonM M Shreeve

Citations

Mar 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G FiorelliM L Brandi
Aug 1, 1995·Microscopy Research and Technique·M Rosendaal
Feb 1, 1985·The Histochemical Journal·J W CattF L Harrison
Dec 2, 2008·International Journal of Hematology·Miwa HebiguchiKenichi Sawada
Feb 5, 2011·International Journal of Hematology·Xiuli An, Narla Mohandas
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·T M Dexter
Jan 1, 1991·Tissue & Cell·M RosendaalC R Green
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·Y Barrandon, H Green
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A S McWilliamS Gordon
Mar 29, 2006·Current Opinion in Hematology·Joel A Chasis
Apr 23, 2013·TheScientificWorldJournal·Jean-Antoine RibeilOlivier Hermine
Jul 25, 2008·Blood·Joel A Chasis, Narla Mohandas
Jun 10, 2011·PloS One·Yu-An CaoChristopher H Contag
Dec 5, 2013·Physiological Reports·Zhenghui WangJohannes Vogel
Feb 24, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jia Hao YeoStuart T Fraser
Jul 22, 2009·IUBMB Life·Asterios S TsiftsoglouJohn Strouboulis
Oct 17, 2014·Immunological Reviews·Siamon GordonFernando Martinez Estrada
Nov 1, 1983·The Journal of Pathology·T M Dexter, T D Allen
Sep 18, 2015·Immunologic Research·Jimmy HomLionel Blanc
Feb 20, 2008·Current Topics in Developmental Biology·Deepa Manwani, James J Bieker
Apr 20, 2017·International Journal of Molecular Sciences·Filippo MolicaBrenda R Kwak
Jan 8, 2009·Toxicologic Pathology·Deidre A DalmasMarshall S Scicchitano
May 19, 2006·The Journal of Biological Chemistry·Shivani SoniManjit Hanspal
Feb 18, 2017·Frontiers in Immunology·Thomas R L KleiRobin van Bruggen
Oct 17, 2017·European Journal of Haematology·Gulnaz T JavanShivani Soni
Mar 20, 2018·Bone·Joshua T Eggold, Erinn B Rankin

Related Concepts

Marrow
Cell Differentiation Process
Erythropoiesis
Mice, Inbred DBA
Electron Microscopy
Scanning Electron Microscopy

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.