Ultrastructural changes in endothelial cells of buffaloes following in-vitro exposure to Pasteurella multocida B:2

BMC Veterinary Research
Y PuspitasariMohd Zamri-Saad

Abstract

Pasteurella multocida B:2 causes haemorrhagic septicaemia in cattle and buffaloes. However, buffaloes are found to be more susceptible to the infection than cattle. Upon infection, the pathogen rapidly spread from the respiratory tract to the blood circulation within 16-72 h, causing septicaemia. So far, limited study has been conducted to evaluate the response of endothelial cells of buffalo towards P. multocida B:2 and its lipopolysaccharide (LPS). This study aimed to evaluate the ultrastructural changes in the aortic endothelium of buffaloes (BAEC) following exposure to P. multocida B:2 and its endotoxin. The endothelial cells were harvested from the aorta of healthy buffaloes and were prepared as monolayer cell cultures. The cultures were divided into 3 groups before Group 1 was inoculated with 107 cfu/ml of whole cell P. multocida B:2, Group 2 with LPS, which was extracted earlier from 107 cfu/ml of P. multocida B:2 and Group 3 with sterile cell culture medium. The cells were harvested at 0, 6, 12, 18, 24, 36, and 48 h post-inoculation for assessment of cellular changes using transmission electron microscopy. The BAEC of Groups 1 and 2 demonstrated moderate to severe endothelial lysis, suggestive of acute cellular injury. ...Continue Reading

References

Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Didier Heumann, Thierry Roger
Feb 3, 2007·Tropical Animal Health and Production·M Zamri-SaadM Y Sabri
Aug 23, 2011·Scandinavian Journal of Immunology·Jeppe Sylvest NielsenJan Krog
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May 17, 2014·Cytotherapy·Forough AtaollahiNoor Azuan Abu Osman
Apr 22, 2018·Microbial Pathogenesis·Sivakumar PeriasamyNem Singh

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Citations

Aug 8, 2021·Animals : an Open Access Journal From MDPI·Elizabeth A ClemmonsJohn W Dutton

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