PMID: 9529858Apr 8, 1998Paper

Ultrastructural changes of Acanthamoeba cyst of clinical isolates after treatment with minimal cysticidal concentration of polyhexamethylene biguanide

The Korean Journal of Parasitology
H H Kong, D I Chung

Abstract

In order to understand the action mechanism of polyhexamethylene biguanide (PHMB) to the cyst of Acanthamoeba on the morphological basis, the cysts of four corneal isolates of Acanthamoeba were treated with minimal cysticidal concentration (MCC) of PHMB and their ultrastructural changes were examined by transmission electron microscopy. The most striking change of cysts treated with PHMB compared with normal cysts was the shrinkage of intracystic amoebae, which resulted in the separation of the plasma membrane of intracystic amoeba from endocystic wall. Subplasmalemmal lipid droplets became irregularly shaped. In severely damaged cysts, cytoplasm was aggregated and organelles were severely deformed. Cytoplasmic materials were leaked out through the damaged plasma membrane. Most cysts showed aggregation of nuclear chromatin material. Number of mitochondrial cristae was also reduced. Ecto- and endo-cystic walls were relatively well tolerated. Findings in the present study revealed that PHMB affected mainly on plasma membrane, but lesser on organellar membrane of intracystic amoeba. It seemed likely that PHMB might kill cystic forms of Acanthamoeba by similar mechanism in which this environmental biocide can damage the cell wall o...Continue Reading

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Citations

Dec 12, 2012·Expert Opinion on Investigational Drugs·Divya Prakash GnanadhasDipshikha Chakravortty
Dec 25, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Fu-Chin HuangWei-Chen Lin
Jul 26, 2015·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Satya Sree N KolarJan P G Bergmanson
Sep 24, 2004·The Korean Journal of Parasitology·Ho-Joon Shin, Kyung-il Im
Jan 18, 2017·The Korean Journal of Parasitology·Eun-Kyung MoonHyun-Hee Kong
Apr 16, 2005·Journal of Korean Medical Science·Hong-Ryul JinTae-Young Yoon
May 26, 2010·Yonsei Medical Journal·Mi Ryung RohKee Yang Chung
Jun 2, 2010·Journal of Korean Medical Science·Hei Sung KimYoung Min Park
Feb 14, 2009·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Regine Bousquet-RouaudAgustina Vila Echague
Jan 22, 2013·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Ajay Kumar VijayMark Duncan P Willcox

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