Jul 1, 1977

Ultrastructural demonstration of cell coat on the cell surfaces of normal human oesophageal epithelium

The Histochemical Journal
K R LoganG Milne

Abstract

The cell coat in human oesophageal biopsies was studied with Alcian Blue, Ruthenium Red, Safranin O, colloidal iron and the ferrocyanide--osmium tetroxide techniques. Alcianophilic material was found on the cell surface of the basal, prickle cell and functional layers, being most abundant on the superficial cells where it appeared as a continuous coat. In the deeper layers, it tended to have a particulate distribution. Some membrane-coating granules were alcianophilic. Ruthenium Red had a particulate distribution over all cell surfaces. Intercellular debris was also stained. Safranin O produced no staining. Colloidal iron stained the cell coat in a particulate manner. The ferrocyanide--osmium technique showed a uniform filamentous cell coat. The oesophageal epithelial cell coats are, in part, acid mucosubstances which, on the surface cells, may have a protective function.

  • References13
  • Citations10

References

  • References13
  • Citations10

Citations

Mentioned in this Paper

Epithelium
Mesothelium
Electron Microscopy
Staining and Labeling
Biopsy
Safranine T
Glycocalyx
Cytochemistry
Prickle Cell of Epidermis
Hexacyanoferrate II

About this Paper

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