PMID: 4293007Sep 1, 1967Paper

Ultrastructural demonstration of cytochrome oxidase activity by the Nadi reaction with osmiophilic reagents

The Journal of Cell Biology
A M SELIGMANJ S Hanker

Abstract

A new method for the subcellular and cytochemical demonstration of cytochrome oxidase has been developed with the introduction of N-benzyl-p-phenylenediamine (BPDA) and the discovery that indoanilines are osmiophilic. These indoanilines produced upon oxidation of BPDA in the presence of naphthols are highly colored compounds that yield electron-opaque coordination polymers of osmium (osmium black) that are amorphous, insoluble in water, and in organic solvents. The best methods for preparing rat tissue were in decreasing order: fixation in formaldehyde solution, fresh tissue slices, and frozen sections of fresh or fixed tissue. Ultrathin sections were counterstained by bridging with the thiocarbohydrazide-osmium tetroxide (T-O) procedure for enhancing underlying membranous structures. Cytochrome oxidase activity was noted primarily in mitochondria and occasionally in sarcotubules of heart, in mitochondria and occasionally in infoldings of the plasma membrane of renal tubular cells, and in mitochondria and, to a great extent, in endoplasmic reticulum of hepatic cells. Cytochrome oxidase activity produced deposits in droplet form, whereas dehydrogenase activity resulted in uniform staining of mitochondrial cristae, as recently de...Continue Reading

References

Jan 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M SeligmanJ S Hanker
Jun 30, 1967·Science·J S HankerA M Seligman
Nov 1, 1958·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M NACHLASA M SELIGMAN
Mar 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M S BURSTONE
Nov 1, 1960·Stain Technology·K C RICHARDSONE H FINKE
Jan 1, 1960·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M S BURSTONE
Jun 1, 1962·The Journal of Cell Biology·J A FREEMAN, B O SPURLOCK
Dec 1, 1961·The Journal of Biophysical and Biochemical Cytology·M J KARNOVSKY
Oct 1, 1961·Journal of Ultrastructure Research·S M KURTZ

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Citations

Jan 1, 1970·Histochemie. Histochemistry. Histochimie·A M SeligmanR E Plapinger
May 1, 1969·The Histochemical Journal·D Hopwood
May 1, 1975·CRC Critical Reviews in Microbiology·P JurtshukW C Acord
May 22, 2010·Progress in Histochemistry and Cytochemistry·Reimar Krieg, Karl-Jürgen Halbhuber
Aug 1, 1968·Experimental Parasitology·A H Rothman
Apr 1, 1971·Experimental Parasitology·S Akao
Apr 12, 2020·Journal of Microscopy·Julie FaitgAmy E Vincent

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