PMID: 6159475Sep 1, 1980Paper

Ultrastructural effects of the high molecular weight cryoprotectants Dextran and polyvinyl pyrrolidone on liver and brown adipose tissue in vitro

Journal of Microscopy
T Barnard

Abstract

The ultrastructural changes caused by incubation of rat liver and brown fat in buffered solutions of high molecular weight cryoprotectives (Dextran and polyvinyl pyrrolidone) at high concentrations (up to 25% w/v) have been examined. Under appropriate conditions of incubation rather small qualitative changes were found. Hepatocytes showed some signs of plasmolysis. Evidence for the endocytosis of Dextran and polyvinyl pyrrolidone was obtained. Cryoultramicrotomy of unfixed, quench-frozen specimens after the same incubation procedures showed good cutting properties and only slight ice-crystal damage. Several of the larger tissue compartments were recognizable in such sections. Although high molecular weight cryoprotectants do penetrate cells by endocytosis (and these effects require further functional evaluation), the present observations provide further support for the idea that such compounds significantly improve the quench-freezing of biological specimens and offer a practical way forward for the preparation of material for X-ray microanalysis of diffusible elements.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·V Herzog, M G Farquhar
Jan 1, 1977·Annual Review of Biochemistry·S C SilversteinZ A Cohn
May 1, 1972·The Journal of Cell Biology·N SimionescuG E Palade

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Citations

Aug 1, 1992·Cryobiology·M DouR Rahmani
Jun 1, 1982·Journal of Microscopy·T Barnard
Apr 1, 1982·Journal of Microscopy·H Y ElderW H Biddlecombe
Jan 1, 1982·Ultrastructural Pathology·G M RoomansL Sevéus
Aug 1, 1982·Journal of Microscopy·D M Harvey

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