PMID: 9164830Jun 1, 1997Paper

Ultrastructural immunolocalization of cartilage oligomeric matrix protein (COMP) in porcine growth cartilage

Calcified Tissue International
S EkmanD Heinegard

Abstract

Cartilage oligomeric matrix protein (COMP) is a macromolecule of yet unknown function with restricted distribution among tissues. In the present study, the ultrastructural localization of COMP in porcine immature joint cartilage and growth plate cartilage was semiquantitatively delineated. Tissues were fixed in a mixture of low concentration glutar- and paraformaldehyde, embedded at low temperature, and subjected to immunocytochemistry using polyclonal antibodies raised against bovine COMP. Protein A-coated colloidal gold was used for detection. The most intense immunolabeling for COMP was noted in the proliferative zones of the growth cartilages. Here the concentration of immunomarker was higher in the territorial compartment than in the pericellular and interterritorial areas. A low concentration of COMP was observed in the resting and hypertrophic zones. The immunolabeling for COMP did not differ between the three matrix compartments of these zones. Supported by previous data obtained by in situ hybridization, the concentration of immunolabeling in the proliferative zone indicates a high rate of COMP synthesis in proliferative chondrocytes. Hence, COMP may be considered as a marker for normal differentiation into proliferati...Continue Reading

Citations

Dec 21, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·E SkiöldebrandS Ekman
Jan 29, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·J T HechtP J Duke
Aug 22, 2002·The Journal of Clinical Investigation·Robert DinserPatrik Maurer
Aug 11, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P S IssackP E Di Cesare
Oct 29, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C FangP E Di Cesare
Jun 9, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Paul S IssackPaul E Di Cesare
Jun 5, 1999·Rheumatic Diseases Clinics of North America·F A Wollheim

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