Ultrastructural immunolocalization of nestin in the regenerating tail of lizards shows its presence during cytoskeletal modifications in the epidermis, muscles and nerves

Tissue & Cell
L Alibardi

Abstract

Nestin has been considered a neural stem cell marker, and represents an intermediate filament protein likely involved in restructuring the cytoskeleton in different cell types. The present ultrastructural study has immunodetected nestin especially in the wound epidermis, regenerating myotubes and in the growing nerves of the regenerating tail of lizards. In keratinocytes of the stratified wound epidermis nestin is present in the irregular electron-paler meshwork located along the cell perimeter and among keratin bundles converging into desmosomes. In the regenerating muscles nestin-immunoreactivity remains confined to some external regions along the myotubes and in the cytoplasmic ends of the myotubes not occupied by myofibrils. A diffuse nestin immunolabeling is also present among the neurofilaments of growing axons, in Schwann cells and in ependymal cells of the regenerating spinal cord of the tail. The localization of nestin in sites of cytoskeletal remodeling in keratinocytes, myotubes, ependymal cells and axons, suggests that this protein is associated to the reassembling of keratin tonofilaments in moving keratinocytes, assembling of contractile proteins in myotubes, and in the organization of neurofilaments during the gr...Continue Reading

References

Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C ScalaG C Manara
Oct 1, 1968·The Journal of Comparative Neurology·S B Simpson
Mar 30, 2001·The Journal of Biological Chemistry·C M SahlgrenJ E Eriksson
Jun 12, 2001·Journal of Neuropathology and Experimental Neurology·S VaittinenH Kalimo
Apr 11, 2003·Molecular Biology of the Cell·Ying-Hao ChouRobert D Goldman
Aug 11, 2004·Nature Cell Biology·Pierre A Coulombe, Pauline Wong
Sep 15, 2005·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Lorenzo Alibardi, Mattia Toni
Jun 1, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hyuno KangWesley Thompson
Aug 19, 2011·BMC Developmental Biology·Katherine E McLean, Matthew K Vickaryous
Oct 1, 2011·Journal of Morphology·Ellen M DawleyKathryn A Matthias
Aug 3, 2012·Acta Dermato-venereologica·Akira WataraiKensei Katsuoka
Nov 13, 2012·Journal of Molecular Histology·Youlang ZhouYan Liu
Jan 9, 2014·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Lorenzo Alibardi

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