Ultrastructural localization of delta-1 opioid receptor in the dorsal raphe nucleus of the rat

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Q-P WangY Nakai

Abstract

The ultrastructural localization of delta-1 opioid receptor in the rat dorsal raphe nucleus was studied by the preembedding avidin-biotin-peroxidase complex technique. With application of a low concentration of the first antiserum in incubation and control of short-time reaction to 3,3'-diaminobenzidine, the immunoreaction seemed to be faint at the light microscopic level. At the electron microscopic level, however, delta-1 opioid receptor immunoreaction products were found to be localized specifically on the postsynaptic membrane of dendrites, dense-cored vesicles, and the surface of the small, clear vesicles in axon terminals with strong immunoreactivity. Of the total 659 immunopositive profiles observed, up to 62.4% (411/659) were dendrites, whereas 33.8% (223/659) were axon terminals. The immunostained myelinated axons and perikarya were relatively rare, with the frequencies 0.8% (5/659) and 3.0% (20/659), respectively. Most of the immunopositive dendrites (338/411, 82.2%) were immunostained only at the postsynaptic membranes. Other immunoreactive dendrites showed their immunoreaction products also in some other contents besides the postsynaptic membranes (44/411, 10.7%) or only in those contents but not the postsynaptic me...Continue Reading

References

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