Ultrastructural localization of glutathione in Cucurbita pepo plants

Protoplasma
M MüllerGünther Zellnig

Abstract

Electronmicroscopic immunogold cytochemistry was used to investigate the cellular and subcellular distribution of glutathione in root and leaf cells of Styrian pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) plants. Gold particles bound to glutathione were found in various cell structures. Statistical evaluation of the gold particle density was made for different cell compartments including nuclei, mitochondria, plastids, peroxisomes, and the cytosol. In each cell type the highest level of glutathione immunoreactivity occurred in mitochondria, for which the labeling density was found to be higher in mesophyll cells of the youngest fully developed leaves (younger leaves) than in the 5th leaves (older leaves) or in root tip cells. Additionally, a statistically significant increase of gold particles bound to glutathione was observed in nuclei (22%) and the cytosol (14%) of the root cells in comparison with mesophyll cells of older (17% and 9%, respectively) and younger leaves (11% and 6%, respectively). The relevance and specificity of glutathione labeling is discussed with respect to difficulties of immunolocalization of low-molecular-weight compounds.

Citations

Feb 27, 2010·Protoplasma·Bernd Zechmann, Maria Müller
Nov 10, 2013·Journal of Experimental Botany·Thierry JoëtStéphane Dussert
Jul 7, 2009·Journal of Plant Physiology·Nieves Fernandez-GarcíaEnrique Olmos
Sep 14, 2007·Plant Biology·H RennenbergS Kopriva
Mar 13, 2015·Frontiers in Plant Science·Francisco J Corpas, Juan B Barroso
Nov 15, 2018·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Julian Oestreicher, Bruce Morgan
Jul 29, 2020·Frontiers in Plant Science·Francisco J CorpasJosé M Palma
Mar 7, 2021·International Journal of Molecular Sciences·Francisco J CorpasJosé M Palma

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