PMID: 6984701Jan 1, 1982Paper

Ultrastructural localization of acetylcholinesterase. A direct method for light and electron microscopy

Histochemistry
J Gautron

Abstract

A method is described allowing localization of acetylcholinesterase (AChE) by both light and electron microscopy. During the reaction lead thio-diacetyl is decomposed, and therefore precipitated as PbS in the presence of native -SH group produced by the hydrolysis of acetylthiocholine perchlorate. The reaction takes place at neutral pH, since improves the sensitivity of AChE localizations. Application of the method to parasympathetic neurons showed that AChE was mainly localized in the rough endoplasmic reticulum of the perikaryons. No reaction was visible in glial cells. AChE was also localized on the plasma membrane of parasympathetic neurons. In mouse embryo muscles AChE activity was seen to be high and was not yet restricted to the synaptic area. The well developed Schwann cells accompanying the neurites displayed constant AChE activity on their plasma membrane.

References

May 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T K GolderB W Wilson
Mar 1, 1977·Journal of Neuropathology and Experimental Neurology·V M TennysonM Brzin
Sep 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H R SawyerB W Wilson
Jul 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V M TennysonL T Kremzner
Dec 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G B Koelle, R S Horn
Dec 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G B KoelleM Devlin
Oct 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R M Friedenberg, A M Seligman
Nov 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M Brzin
Nov 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W A KoelleG B Koelle
Apr 1, 1966·The Journal of Experimental Zoology·E A Maynard
Jul 1, 1953·The Biochemical Journal·L AUSTIN, W K BERRY
Jun 1, 1954·British Journal of Pharmacology and Chemotherapy·M P FULTON, G A MOGEY
Sep 23, 1955·Science·M CREVIER, L F BELANGER
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS
Apr 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·G B KOELLE, J A FRIEDENWALD

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Citations

Oct 23, 1997·The Journal of Comparative Neurology·A Czupryn, J Skangiel-Kramska
Apr 1, 1992·Journal of the Neurological Sciences·G L ForloniA Vezzani
Jul 1, 1992·Molecular and Biochemical Parasitology·C C Blackburn, M E Selkirk
Aug 31, 1994·Journal of Biotechnology·C Schmidt-DannertR D Schmid
Jan 1, 1986·General Pharmacology·Y T DasS K Chattopadhyay
Mar 7, 2002·Journal of Neuroimmunology·Chi Mei HsuehHsin Ju Chao
Jan 1, 1995·Progress in Histochemistry and Cytochemistry·M Bendayan
Nov 11, 2009·Carbohydrate Research·Goreti Ribeiro MoraisRobert A Falconer
May 2, 2003·The Journal of Comparative Neurology·Paolo BurighelLucia Manni
Nov 13, 2013·Muscle & Nerve·Padmamalini Baskaran, Baskaran Thyagarajan
Dec 3, 2014·European Journal of Pharmacology·Baskaran ThyagarajanJoseph J McArdle
Jul 3, 2013·Toxicon : Official Journal of the International Society on Toxinology·Padmamalini BaskaranBaskaran Thyagarajan
Dec 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bendayan, V Gisiger
Jun 29, 2012·American Journal of Physiology. Endocrinology and Metabolism·Carmen C GarciaJoseph J McArdle

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