Ultrastructural responses of pancreatic beta cells to metabolic alkalosis

Cell and Tissue Research
C L Gómez DummJ J Gagliardino

Abstract

The ultrastructural changes in pancreatic beta cells were studied following glucose-induced insulin secretion in vitro, at two different extracellular pH (7.4 and 7.8). The pancreata perfused at pH 7.4 exhibited a biphasic insulin response to glucose challenge together with signs of increased emiocytotic activity and numerous microtubules in the beta cells. Conversely, the pancreata perfused at pH 7.8 showed a significant decrease in insulin secretion, and their beta cells revealed scarce emiocytotic images and a marked increase of intracellular granulolysis. These results represent the ultrastructural correlate of the reduced insulin secretion produced by metabolic alkalosis in the perfused rat pancreas.

Related Concepts

Alkalosis
Metazoa
Anhydrous Dextrose
Hydrogen-Ion Concentration
Insulin B Chain
Islets of Langerhans
Microtubules
Perfusion
Rats, Laboratory
In Vitro [Publication Type]

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