Ultrastructural responses of pancreatic beta cells to metabolic alkalosis

Cell and Tissue Research
C L Gómez DummJ J Gagliardino


The ultrastructural changes in pancreatic beta cells were studied following glucose-induced insulin secretion in vitro, at two different extracellular pH (7.4 and 7.8). The pancreata perfused at pH 7.4 exhibited a biphasic insulin response to glucose challenge together with signs of increased emiocytotic activity and numerous microtubules in the beta cells. Conversely, the pancreata perfused at pH 7.8 showed a significant decrease in insulin secretion, and their beta cells revealed scarce emiocytotic images and a marked increase of intracellular granulolysis. These results represent the ultrastructural correlate of the reduced insulin secretion produced by metabolic alkalosis in the perfused rat pancreas.

Related Concepts

Anhydrous Dextrose
Hydrogen-Ion Concentration
Insulin B Chain
Islets of Langerhans
Rats, Laboratory
In Vitro [Publication Type]

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