Ultrastructural studies in passive in situ immune complex glomerulonephritis. A rat model for membranous glomerulonephritis.

Virchows Archiv. B, Cell Pathology Including Molecular Pathology
M SasakiA Vogt

Abstract

Morphologic studies were performed in a passive model of in situ immune complex glomerulonephritis in rats. The formation and fate of subepithelial immune complexes as well as the role of glomerular polyanion in the induction of disease were examined. Unilateral in situ immune complex glomerulonephritis was induced in rats by perfusion of cationised horse spleen ferritin (pI greater than 9.5) (400 micrograms/rat) into the left kidney followed by systemic injection of 0.2 ml (= 400 micrograms precipitating antibody) of sheep anti-ferritin antiserum 2 h later. This schedule induced glomerulonephritis with proteinuria (mean maximum 100 mg/24 h between the 5th and the 12th day). Rats were sacrificed at intervals between 1 h and 42 days after induction of glomerulonephritis, samples of renal tissue were examined by light, immunofluorescence and electron microscopy (including staining of anionic sites by polyethyleneimine). The lesion induced closely resembled that of membranous glomerulonephritis in man as massive subepithelial deposits were seen with very little cellular infiltration or proliferation. The antigen (ferritin) deposits were initially located subepithelially; from 2 weeks onwards intramembranous deposits in the thicken...Continue Reading

References

Aug 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J W SchurerI Molenaar
Sep 1, 1988·Kidney International·J W FriesH G Rennke
Jan 1, 1983·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·Y SuzukiM Arakawa

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Citations

Jan 29, 2000·Kidney International·C J RaatsJ H Berden
Jun 1, 1990·Irish Journal of Medical Science·A GreenJ Donohoe
Aug 26, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·M RomeroG Parra

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