Ultrastructural studies of the bone marrow in sickle cell anaemia. II. The morphology of erythropoietic cells and their response to deoxygenation in vitro

British Journal of Haematology
J A GrassoL W Sullivan

Abstract

Electron microscopic studies of bone marrow aspirates obtained from patients with homozygous sickle cell anaemia (HbSS) were fixed immediately without attempts to deoxygenate the samples. Erythroblasts and normoblasts in these preparations were devoid of haemoglobin polymers or other indications of sickling. Furthermore, the nucleated erythroid cells from sickle-cell patients presented an ultrastructural morphology indistinguishable from that of identically-processed erythroid cells in marrow samples from normal human volunteers. This report presents a description of the ultrastructural features of pronormoblasts and normoblasts in normal and sickle-cell marrows and stresses the essentially normal appearance of nucleated erythroid elements in sickle cell anaemia. Exposure of sickle-cell marrow aspirates to nitrogen at 37 degrees C for 30 min resulted in haemoglobin polymerization in most erythrocytes and reticulocytes but only in 10-20% of the nucleated erythroid cells. Haemoglobin polymers in the form of intertwining fibre meshworks were observed in reticulocytes, orthochromatic and polychromatophilic normoblasts, but were absent in basophilic normoblasts and pronormoblasts. The results suggest that the concentration of haemog...Continue Reading

References

Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J HofrichterW A Eaton
Mar 1, 1973·British Journal of Haematology·A Polliack, E A Rachmilewitz
Feb 1, 1974·British Journal of Haematology·A PolliackE A Rachmilewitz
Jul 1, 1969·British Journal of Haematology·J A Grasso, J D Hines
Dec 1, 1966·The Anatomical Record·J A Grasso
Feb 1, 1967·Journal of Ultrastructure Research·I Berman
Jun 1, 1967·The Journal of Cell Biology·E Skutelsky, D Danon
Sep 23, 1961·JAMA : the Journal of the American Medical Association·J F JACKSONW N BELL
Aug 1, 1962·The Journal of Cell Biology·J A GRASSOG A ACKERMAN
Sep 1, 1964·The Journal of Cell Biology·R A RIFKINDP A MARKS

❮ Previous
Next ❯

Citations

Oct 18, 2016·Stem Cells and Development·Marina CavazzanaIsabelle André-Schmutz
Apr 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·S W Tang, P Seeman
Jan 1, 1982·Clinical and Laboratory Haematology·S N WickramasingheM Hughes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.