PMID: 8813078Oct 1, 1996Paper

Ultrastructural study of degradation of calcium phosphate ceramic by human monocytes and modulation of this activity by HILDA/LIF cytokine

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
M BenahmedG Pradal

Abstract

Biodegradation of ceramics in vivo is achieved essentially by monocytes and multinuclear cells (osteoclasts). Monocytes are the key element in this process because they intervene first at the biomaterial implantation site during inflammatory reaction. In this work, in vitro studies were conducted on an ultrastructural scale to determine the specific behavior of these cells with regard to a calcium phosphate (CaP) ceramic. Two types of phagocytosis were observed when cells came into contact with the biomaterial: either CaP crystals were taken up alone and then dissolved in the cytoplasm after disappearance of the phagosome membrane or they were incorporated together with large quantities of culture medium, in which case dissolution occurred after the formation of heterophagosomes. Phagocytosis of CaP coincided with autophagy and the accumulation of residual bodies in the cells. Addition of HILDA/LIF factor to these cultures induced a very marked decrease in phagocytotic activity directed at the capture of CaP crystals and culture medium. Autophagy was reduced, and residual bodies were rare or absent. This study specifies the role of monocytes in CaP biodegradation and demonstrates for the first time that HILDA/LIF has a biologic...Continue Reading

Citations

Sep 16, 2014·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Philip M FinchPeter D Drummond
Sep 8, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·R de WaterD J Kok
Oct 12, 2000·Cytokine·D Heymann, A V Rousselle
Apr 5, 2013·Journal of Biomedical Materials Research. Part a·Gerald ZimmerOskar Hoffmann
Jun 3, 2016·Journal of Biomedical Materials Research. Part a·Kevin BrulefertD Heymann
Jan 23, 2019·Journal of Biomaterials Applications·Fan LiuIn-Seop Lee
Jan 27, 2019·Journal of Biomedical Materials Research. Part a·Yukari ShiwakuOsamu Suzuki

Related Concepts

LIF protein, human
Bioremediation
Cell Differentiation Process
Ceramics
Foreign-Body Reaction
Growth Inhibitors
Cricetus
Lymphokines
Macrophage
Electron Microscopy

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