Ultrastructural study of development of hepatic necrosis induced by TNF-alpha and D-galactosamine

Digestive Diseases and Sciences
K TakenakaK Okita

Abstract

Recent studies have suggested an association between tumor necrosis factor-alpha (TNF-alpha) and the development and progression of acute liver failure. To investigate the role of TNF-alpha in the mechanism of massive hepatic necrosis, we studied a mouse model of TNF-alpha and D-galactosamine (GalN) -induced hepatic necrosis by ultrastructural analysis. Administration of GalN caused edema of hepatocellular microvilli and widening of sinusoidal endothelial fenestrae (SEF); administration of TNF-alpha caused only a widening of the SEF. Massive hepatic necrosis with hemorrhage was seen 6 hr after concomitant administration of TNF-alpha and GalN. In the ultrastructural analysis, edema of the hepatocellular microvilli, widening of the SEF, and transmigration of red blood cells (RBC) and platelets to the space of Disse without exfoliation and necrosis of the sinusoidal endothelial cells were observed. Fibrin deposits were seen in areas adjacent to injured hepatocytes. The diameter of the SEF was significantly greater than in the nontreated group and the groups treated with TNF-alpha or GalN alone. These results suggest that as a consequence of the increase in diameter of the SEF, transmigration of RBCs and platelets to the space of D...Continue Reading

Citations

Mar 1, 2011·Experimental and Therapeutic Medicine·Kazuhiro KotohRyoichi Takayanagi
Jul 9, 2004·World Journal of Gastroenterology : WJG·Yue GaoHong-Chi Jiang
Apr 1, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yoshiya ItoRobert S McCuskey
Dec 8, 2011·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Ann-Marie T BaineJustin H Nguyen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.