Ultrastructural study on nephrin expression in experimental puromycin aminonucleoside nephrosis

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Young Ki LeeHirashi Kawachi

Abstract

Nephrin is a recently identified protein that is a key component of the slit diaphragm. This protein may play a crucial role in maintaining the glomerular filtration barrier, and mutations in the gene for nephrin reportedly lead to congenital nephrosis. However, the expression of nephrin in acquired glomerular disease has not yet been fully clarified. To address this issue, we analysed the expression and localization of nephrin by morphological analysis based on immunoelectron microscopy in normal glomeruli and in glomeruli from proteinuric experimental models. Twenty rats were divided into three experimental groups (n = 16 total) and a control group (n = 4). Rats in the experimental groups received a single intravenous injection of puromycin aminonucleoside (PAN), and were sacrificed at 1 (n = 4), 2 (n = 6) and 3 weeks (n = 6) post-injection. Nephrin expression was assessed by immunoelectron microscopy using a polyclonal antibody against nephrin and gold particles. It was quantified by counting the gold particles and the slit diaphragms and by measuring the average foot process width in microphotographs. The average foot process width in the 1 week group (5924.5 +/- 1523.9 nm) was far greater than that of controls (1112.9 +/- ...Continue Reading

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Citations

Aug 5, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Tomonosuke SomeyaYuichiro Yamashiro
Aug 25, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Hirotaka KawakamiKinji Shirota
May 12, 2016·Kidney International·Michio Nagata
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Jan 13, 2019·Journal of the American Society of Nephrology : JASN·Yasuhiro YoshimuraRyuichi Nishinakamura
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Feb 5, 2015·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Yu ShengyouMa Yuanyuan
Apr 26, 2006·Experimental Biology and Medicine·Yan XingNa Guan

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