Ultrastructure and cytochemical staining characteristics of canine natural killer cells

The Anatomical Record
D W KnappJ P Robinson

Abstract

The purpose of this work was to describe the ultrastructure and cytochemical staining characteristics of canine peripheral blood lymphocytes with natural killer (NK) cell activity, with comparison made to non-NK lymphocytes. Canine lymphocyte populations evaluated for ultrastructure, cytochemical staining, and NK function (by 51chromium release assay) included: peripheral blood lymphocytes; lymphocytes from band 1 (NK-enriched), band 2, and the pellet of a 45/50% percoll gradient; lymphocytes from the supernatant fluid (non-conjugated lymphocytes) and pellet (lymphocytes conjugated to tumor cell targets) of a 17% percoll gradient; and null (CD4-CD8-) and CD4-CD8+ lymphocytes. NK activity was concentrated in band 1 lymphocytes of the 45/50% percoll gradient with further enhancement of activity occurring in sorted null cells. Canine NK cells were 5.5 to 6.5 microns in diameter with a reniform (kidney bean shape) nucleus, and electron-dense cytoplasmic granules. NK cells (percoll band 1 cells and null cells) had larger cell and nuclear area, and less round nuclei when compared to non-NK lymphocytes. The overall cytochemical staining (chloracetate esterase, peroxidase, sudan black B, naphthyl acetate esterase, naphthyl butyrate est...Continue Reading

References

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Citations

Apr 4, 2013·Veterinary Immunology and Immunopathology·Dong-Jun ShinSang-Ki Kim
Aug 28, 2009·Veterinary Immunology and Immunopathology·Yi-Chun LinRea-Min Chu
Apr 22, 2008·Veterinary Immunology and Immunopathology·R Curtis BirdBruce F Smith
Dec 2, 2014·Veterinary Research Communications·Christine Grøndahl-RosadoAnne K Storset
Jul 21, 2016·Veterinary Immunology and Immunopathology·Christine Grøndahl-RosadoAnne K Storset
Sep 19, 2002·The Journal of Veterinary Medical Science·Masato KuwabaraMasayoshi Yukawa

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