PMID: 6163958Feb 11, 1981Paper

Ultrastructure and polysome content of the microsomal subfractions of mouse plasmacytoma cells

Molecular and Cellular Biochemistry
A M SvardalH Dalen

Abstract

The endoplasmic reticulum (ER) of MPC-11 cells released as vesicles upon cell disruption by nitrogen cavitation was separated from the bulk of mitochondria, lysosomes and plasma membranes by a low speed centrifugation. The ER membranes were fractionated on discontinuous sucrose gradients into heavy rough (HR), light rough (LR) and smooth (S) membranes. The morphology of subcellular fractions was studied by electron microscopy and the ER membranes were shown to be virtually free of contaminating organelles. The S fraction was easily distinguishable because of the lack or ribosomes but there were no apparent morphological differences between the HR and LR fractions. Of total activity in the microsomal subfractions, 70% of the UDPase and 67% of the 5'-nucleotidase activity was associated with the S fraction. Polysomes were present in the HR, LR and nuclear-associated ER fractions but not in the S fraction. The HR and LR fractions did not appear to be contaminated to any great extent with free polysomes. RNA/protein and RNA/phospholipid ratios of the HR fraction were higher than those of the LR fraction, indicating a greater density of ribosomes in the former fraction. These ratios were much lower in the S fraction reflecting the l...Continue Reading

References

Dec 29, 1975·Biochimica Et Biophysica Acta·J W Depierre, G Dallner
Oct 26, 1976·Biochimica Et Biophysica Acta·P R McIntosh, K O'Toole
Nov 1, 1973·Experimental Cell Research·K A AbrahamR M Dowben
Aug 24, 1970·Biochemical and Biophysical Research Communications·H Hartman, R M Dowben
Sep 16, 1974·European Journal of Biochemistry·T M HarrisonC Milstein
Mar 1, 1973·European Journal of Biochemistry·P J Birckbichler, I F Pryme
Mar 1, 1973·European Journal of Biochemistry·I F PrymeR M Dowben
Oct 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Dallner, L Ernster
Jul 9, 1971·Nature·C Faiman, J S Winter
Jun 21, 1971·Annals of the New York Academy of Sciences·M Goldman
Jan 1, 1971·Protoplasma·W W Franke, J Kartenbeck
May 9, 1972·Biochimica Et Biophysica Acta·E FerberW Imm
Nov 1, 1969·Experimental Cell Research·R M DowbenD Y Hsia
Jan 1, 1980·Sub-cellular Biochemistry·A M Svardal, I F Pryme
Nov 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ
Jul 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L WATSON
Mar 4, 1961·Biochimica Et Biophysica Acta·M J HUNTER, S L COMMERFORD
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT
Aug 1, 1961·Journal of Ultrastructure Research·B F TRUMPE P BENDITT

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