PMID: 11911985Mar 26, 2002Paper

Ultrastructure of alpha-synuclein-positive aggregations in U373 astrocytoma and rat primary glial cells

Neuroscience Letters
N StefanovaM Reindl

Abstract

Abnormal alpha-synuclein-positive glial cytoplasmic inclusions are found in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. We have recently developed an in vitro model of alpha-synuclein-immunoreactive aggregations in U373 astrocytoma cells. We have additionally overexpressed wild-type and a C-terminally truncated form of alpha-synuclein in primary rat glial cells. Astrocytes and oligodendrocytes were found to form alpha-synuclein-positive aggregations in vitro perinuclearly or in the processes of the cells. The morphological studies presented here demonstrate that the aggregations we have observed in vitro are not limited by a membrane but have unclear borders. They have an amorphous dense core that is intensely alpha-synuclein-immunopositive and a predominantly filamentous halo around. Mainly filamentous structures at the border area between the halo and the core are alpha-synuclein-immunoreactive. We conclude that this in vitro model of alpha-synuclein-positive glial aggregations mimics the morphology of the abnormal glial inclusions described in neurodegenerative disorders and could be a suitable model for studying their role in the pathogenesis of these diseases.

References

Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Jul 14, 1998·Nature Medicine·E MezeyM J Brownstein
Sep 16, 1998·Journal of Neuropathology and Experimental Neurology·S Probst-CousinF Gullotta
Sep 6, 2001·Journal of Neuroscience Research·N StefanovaM Reindl

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Citations

Jun 20, 2003·Neuropharmacology·Kumlesh K DevHerman van der Putten
Sep 23, 2003·Movement Disorders : Official Journal of the Movement Disorder Society·Gregor K WenningFrançois Tison
Jul 18, 2003·Journal of Neuroscience Research·Nadia StefanovaMarkus Reindl
Aug 11, 2005·Movement Disorders : Official Journal of the Movement Disorder Society·Nadia StefanovaGregor K Wenning
Nov 18, 2009·Journal of Neuropathology and Experimental Neurology·Yun Ju C SongTamas R Revesz
May 20, 2020·The Journal of Biological Chemistry·Zachary A Sorrentino, Benoit I Giasson
Sep 26, 2020·Frontiers in Neurology·Pauline MenckeRejko Krüger
Aug 2, 2011·The American Journal of Pathology·Nadia StefanovaGregor K Wenning
Oct 7, 2021·Journal of Neural Transmission·David J MarmionJeffrey H Kordower

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