PMID: 4898837Oct 1, 1969Paper

Ultrastructure of papain and pepsin digestion fragments of human IgM globulins

The Journal of Experimental Medicine
S E SvehagM Seligmann

Abstract

The ultrastructure of papain and pepsin-digested products of human IgM globulins has been analyzed. Papain digestion was performed both in the presence and absence of cysteine. The Fcmicro fragment was found to represent the central ring structure in the intact IgM molecule, plus a minor part of the appendages extending from the ring. The Fcmicro ring structure was occasionally seen to be composed of dimers of short rods, probably identical with the endpieces of two micro-chains. Such dimeric structures, released from the intact Fcmicro rings, had a tendency to aggregate sidewise, producing complexes of varying size. The dimensions of the Fcmicro fragments were: outer diameter approximately 85 A, inner diameter about 40 A. The length of the protrusions varied from 20-30 A. The Fabmicro preparations contained long strands of sidewise aggregated, short rod-shaped fragments. No aggregates were seen in the F(ab'')(2)micro preparations. The two Fab''micro units in the dimeric F(ab'')(2)micro fragments were usually parallel to each other. The dimensions of the Fabmicro and F(ab'')(2)micro fragments were 50-80 A x 30 A and 75-80 A x 55 A, respectively. These findings provide morphological evidence that the C-terminal ends of the micro...Continue Reading

References

May 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·H Metzger
Aug 14, 1967·Journal of Molecular Biology·R C Valentine, N M Green
Aug 15, 1967·Biochimica Et Biophysica Acta·H Ungar-WaronM Sela
Mar 1, 1968·The Journal of Experimental Medicine·B ChesebroS E Svehag
Mar 1, 1968·The Journal of Experimental Medicine·C Mihaesco, M Seligmann
Jun 1, 1965·Immunochemistry·J D AlmeidaD Naylor
Nov 1, 1966·The Journal of Experimental Medicine·T Suzuki, H F Deutsch
May 1, 1968·The Journal of Experimental Medicine·M M Frank, J H Humphrey
Dec 9, 1968·Biochemical and Biophysical Research Communications·E Mihaesco, C Mihaesco
Jul 1, 1959·Biochimica Et Biophysica Acta·S BRENNER, R W HORNE

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Citations

Jul 1, 1970·Immunochemistry·K J Dorrington, C Mihaesco
Jan 1, 1977·Immunochemistry·C RivatC Ropartz
Dec 31, 1971·Annals of the New York Academy of Sciences·A FeinsteinN E Richardson
Jul 1, 1973·European Journal of Immunology·J Zikán, J C Bennett

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