PMID: 3748279Jul 1, 1986Paper

Ultrastructure of reconstituted membranes containing the muscarinic receptor

Neurochemical Research
A Pellegrino de IraldiE L Ochoa

Abstract

In this paper the demonstration is made that membrane vesicles (liposomes) containing the muscarinic receptor can be formed by polyethylene glycol (PEG) precipitation of detergent extracts of bovine atrial membranes. The incorporation of the muscarinic receptor in these vesicles may be related to the restoration of the heterogeneity and nucleotide modulation of muscarinic agonist binding by PEG precipitation of atrial detergent extracts, previously reported. Vesicles are also formed when detergent solubilized asolectin lipids, alone or in combination with membrane detergent extracts, are precipitated by PEG. The structure of the vesicles seems depend on their lipid and protein composition and the procedure employed for the removal of the dispersing medium. These results indicate that PEG precipitation could be used for the reconstitution of the muscarinic receptor into the liposomes of exogenous lipids.

References

Oct 19, 1979·Biochimica Et Biophysica Acta·C P Tilcock, D Fisher
Sep 15, 1976·The Biochemical Journal·B MaggioJ A Lucy
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G B WarrenJ C Metcalfe
Feb 1, 1984·European Journal of Biochemistry·R Kremenetzky, D Atlas
Jan 1, 1984·The Journal of Membrane Biology·L T BoniS W Hui
Feb 9, 1983·Biochimica Et Biophysica Acta·K ArnoldK Gawrisch
Jan 1, 1980·Annual Review of Biophysics and Bioengineering·F Szoka, D Papahadjopoulos
Jan 1, 1981·The Journal of Membrane Biology·L T BoniS W Hui

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Citations

Jan 1, 1987·Neurochemical Research·J S AguilarE De Robertis
Jan 1, 1987·Neurochemistry International·J Santiago Aguilar, E De Robertis
Jul 1, 1992·European Journal of Pharmacology·J E ValliereS M Shreeve
Dec 19, 2009·Analytical Biochemistry·Frank TalmontJean-Marie Zajac

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