Ultrastructure of the Corynebacterium glutamicum cell wall

Antonie van Leeuwenhoek
S MarienfeldA Burkovski

Abstract

The cell wall structure of the Gram-positive Corynebacterium glutamicum was evaluated by electron microscopy of thin sections after freeze-substitution and conventional fixation with glutaraldehyde. For the cell wall an overall thickness of approximately 32 nm was determined, with 8.5 nm corresponding to an outer layer, 6.5 nm to an electron translucent region (ETR) as found in mycobacteria and 17 nm to the peptidoglycan. Knob-like surface structures previously observed in freeze-fracture experiments were detected when cells were conventionally processed with a fixation using glutaraldehyde. By mild treatment with detergents approximately 20 proteins were extracted from the cell wall. From seven of these N-terminal amino acid sequences were determined.

Citations

Jun 26, 2008·The Journal of Biological Chemistry·Matthew J FooterJulie A Theriot
Apr 9, 2010·Applied Microbiology and Biotechnology·Kevin Michael SmithJames C Liao
Jun 1, 2013·ISRN Microbiology·Andreas Burkovski
Jun 27, 2001·Electrophoresis·T HermannA Burkovski
Oct 28, 2017·Archives of Microbiology·Natalia Maria TheresiaMasaaki Wachi

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