Ultraviolet B-induced apoptosis of human skin fibroblasts involves activation of caspase-8 and -3 with increased expression of vimentin

Photodermatology, Photoimmunology & Photomedicine
Haoxiang XuBaoxi Wang

Abstract

After irradiation with a high dose of ultraviolet B (UVB), cells undergo apoptosis. Caspase-8 and -3 are key mediators of apoptosis in many cells. Vimentin, an important cytoskeleton component, can be cleaved by caspase-3, -6, -7 and -8. Cell apoptosis is promoted via caspase-triggered proteolysis of vimentin. In this study, we explored the roles of caspase-8 and -3 and the changes in vimentin expression in UVB-induced apoptosis of human dermal fibroblasts. Skin fibroblasts were irradiated with 150 mJ/cm(2) UVB and cell death was monitored by the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide assay and Hoechst staining. Caspase-8 and -3 activities were detected by the caspase activity assay. Vimentin expression was assessed by immunofluorescence and Western blot. Caspase-8 and -3 were activated by 150 mJ/cm(2) UVB irradiation. Caspase-8 and -3 activities changed in a time-dependent way after UVB irradiation to induce apoptosis of fibroblasts, and caspase-8 and -3 interacted with each other in this process. However, their substrate, vimentin, showed an enhanced expression over time after UVB irradiation. UVB-triggered apoptosis of fibroblasts was dependent on the activation of caspase-8 and -3 with an increased ex...Continue Reading

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Citations

Sep 29, 2011·Archives of Dermatological Research·Yun Kyung LeeSungkwan An
Oct 15, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Konrad KleszczyńskiTobias W Fischer
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Nov 26, 2010·Photodermatology, Photoimmunology & Photomedicine·Yan YanBaoxi Wang
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Mar 17, 2017·PloS One·Marie-Catherine Drigeard DesgarnierPatrick J Rochette
Nov 24, 2020·Stem Cell Research & Therapy·Shidie ChenJinghong Xu

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