PMID: 9558489Apr 29, 1998Paper

Ultraviolet B radiation increases steady-state mRNA levels for cytokines and integrins in hairless mouse skin: modulation by topical tretinoin

Archives of Dermatological Research
E SchwartzL H Kligman

Abstract

Chronically sun-damaged human skin has a wrinkled, aged appearance as a result of alterations in the dermal extracellular matrix. Secondary effectors such as cytokines and integrins may mediate the effects of UV radiation on the skin by regulating the synthesis of metalloproteinases and structural proteins including collagen. The aim of this study was to semi-quantify the steady-state mRNA levels of interleukin-1 alpha, tumor necrosis factor alpha, transforming growth factor beta, collagenase, stromelysin, collagen, and integrins (alpha, and alpha2) in the skin of hairless mice that were either treated with UV or concurrently treated with UV and topical tretinoin for 5 weeks. Total RNA was extracted from the skin of the mice, reverse transcribed to cDNA, and amplified by the polymerase chain reaction in the presence of 32P-dCTP using gene-specific primers. Results were normalized relative to glyceraldehyde-3-phosphate dehydrogenase levels. Steady-state mRNA levels of the cytokines and integrins were increased by UV radiation. Concurrent UV and topical tretinoin treatment superinduced the expression of interleukin-1, increased alpha 1, and decreased alpha 2 integrin expression. Immunofluorescence analysis showed increased dermal...Continue Reading

Citations

Apr 15, 2000·Journal of Dermatological Science·S Kondo
Mar 1, 2012·Journal of Cosmetic Dermatology·Philippe HumbertPatrice Muret
Nov 13, 2013·Biochemical and Biophysical Research Communications·Yuko Matsuura-HachiyaToshio Nishiyama
May 23, 2014·American Journal of Clinical Dermatology·Supenya Varothai, Wilma F Bergfeld
Aug 4, 2019·Photodermatology, Photoimmunology & Photomedicine·Hiroyasu IwahashiHitoshi Masaki

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